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  • E8897 - Monoclonal Anti-Ezrin antibody produced in mouse

E8897 Sigma

Monoclonal Anti-Ezrin antibody produced in mouse

clone 3C12, ascites fluid



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell Membrane, Antibodies to Cell and Organelle Proteins,
species reactivity   bovine, human, monkey, kangaroo rat, hamster, mouse
application(s)   immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunoprecipitation: suitable
  microarray: suitable
  western blot: 1:4,000 using cultured human fibroblast cell line extract
clone   3C12, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 80 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... EZR(7430)
mouse ... Ezr(22350)
rat ... Ezr(54319)
biological source   mouse
conjugate   unconjugated



carboxy-terminal section of recombinant human ezrin (amino acids 362-585).

General description

Ezrin is a member of the best studied member of the ERM family of proteins. The ERM family regulates the dynamic cell membrane functions such as endocytosis, exocytosis, and transmembrane and other cortical signalling pathways. Ezrin (molecular weight 80 kDa) interacts with transmembrane proteins and F-actin and mediate important signalling pathways including RhoA and Hedgehog signalling. Ezrin is also present in the microvillus surface of acid-secreting parietal cells of the gastric glands, where it presents as a family of isoelectric variants. By immunoelectron microscopy of human choriocarcinoma cells, ezrin is enriched just inside the plasma membrane of microvilli and less abundant on more planar aspects of the membrane.


The antibody was used for detection of Ezrin by immunostaining, immunoblotting in human bladder cancer cells , immunohistochemistry, immunoprecipitation in kidney and colon adenocarcinoma cells and electron microscopy in astrocytes

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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