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E9026 Sigma

Anti-E2F1 antibody produced in rabbit

~0.8 mg/mL, affinity isolated antibody, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Gene Regulation, Antibodies to Common Transcription Factors, Antibodies to Transcription Factors,
species reactivity   human
application(s)   immunoprecipitation: suitable
  microarray: suitable
  western blot: 2.5 μg/mL using whole extract of cells expressing recombinant human E2F1 or in vitro translated E2F1 protein
clone   polyclonal
concentration   ~0.8 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 53 kDa (may vary in a recombinant preparation)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... E2F1(1869)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide (amino acid residues 310-329) corresponding the C-terminal region of human E2F1.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

Anti-E2F1 antibody produced in rabbit is suitable for the following applications:
• Immunoprecipitation
• Microarray
• Western blotting at a concentration of 2.5μg/mL using whole extract of cells expressing recombinant human E2F1 or in vitro translated E2F1 protein

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Biochem/physiol Actions

Transcription factor E2F1 is a protein encoded by the E2F1 gene in humans and belongs to E2F family of transcription factors. It is located on chromosome 20q11 and plays a critical role in the transcription of certain genes required for cell cycle progression. E2F1 may facilitate identification of additional E2F family members and detection of E2F1 abnormalities in human tumors. It plays a crutial role in regulating cell cycle and other biological processes in cells. E2F-1 gene expression is higher in rectal cancer tissue compared to normal tissue.

Price and Availability

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