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E9277 Sigma

EphB1/Fc Chimera from rat

>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

Synonym: Cek6, Elk, Hek6, Net

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Properties

Related Categories Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes, Cell Signaling and Neuroscience,
recombinant   expressed in NSO cells
assay   >95% (SDS-PAGE)
form   lyophilized powder
mol wt   monomer calculated mol wt 85.6 kDa
  mol wt ~110 kDa by SDS-PAGE (reducing)
impurities   endotoxin, tested
storage temp.   −20°C
Gene Information   rat ... Ephb1(24338)

Description

Analysis Note

The biological activity is measured by its ability to bind recombinant mouse ephrin-B1/Fc in an ELISA assay.

Biochem/physiol Actions

Member of Eph receptor tyrosine kinase family shown to bind ephrin-A1, ephrin-A3, ephrin-A4, ephrin-B1, ephrin-B2, and ephrin-B3; involved in pattern formation and morphogenesis. EphB1 can be detected in endothelial cells and is activated by ephrin-B1, initiating the assembly of endothelial cells into capillary-like cords.

Ephb1 (Epherin receptor B1) signaling is essential for cell assembly and attachment, mediated through αvβ3 and α5β integrins. It prevents the mixing of second and third neural crest, and is essential for the correct guiding of third neural crest. In retina, it is involved in the formation of ipsilateral projections and axon divergence. It also acts as an activator of c-Jun Kinase (JNK) signaling, through its interaction with the adaptor protein Nck (non-catalytic region of tyrosine kinase).

Other Notes

Extracellular domain of rat EphB1 (amino acids 18-538) fused to the C-terminal 6X histidine-tagged Fc region of human IgG1 via a polypeptide linker.

Physical form

Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, pH 8.5.

Application

EphB1/Fc Chimera from rat has been used for the investigation of the role of ephrinB-EphB signaling in the induction and maintenance of hyperalgesia, hyperexcitability of neurons of DRG (dorsal root ganglion) and hyperexcitability and elevated synaptic plasticity of neurons of DH (dorsal horn) regions of brain.

General description

Ephb1 (Epherin receptor B1) is a receptor tyrosine kinase (RTK), which is a part of the Eph family. It has a leader sequence at its N-terminal, an extracellular domain rich in cysteine, a transmembrane region, a tyrosine kinase domain in its cytoplasmic region and a C-terminal tail. In rats, its expression is exclusive to brain and testis, with higher expression in brain.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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