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E9640 Sigma

Endothelial cell growth factor

100 ×, sterile-filtered, suitable for cell culture

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Properties

Related Categories Cell Culture, Endothelial Cells, Human Umbilical Vein Endothelial Cell (HUVEC) Culture Companion Products and Supplements, Primary Human Cell Culture, Specialty Media Systems More...
sterility   sterile-filtered
concentration   100 ×
impurities   endotoxin, tested
suitability   suitable for cell culture
shipped in   dry ice
storage temp.   −70°C

Description

Preparation Note

5 mg/ml each porcine heparin and HBGF-1 in DPBS without calcium and magnesium

Application

Endothelial Cell Growth Factor (ECGF) has been optimized for the culture of Human Umbilical Vein Endothelial Cells (HUVEC). ECGF is optimized for the mitogenic proliferation of HUVEC cells when supplemented to CS-C Medium. ECGF should be supplemented to CS-C medium at a concentration 1 ml per 100 ml of culture medium. ECGF is prepared in DPBS (without calcium and magnesium) supplemented with porcine heparin and HBGF-1. For best results it is recommended that this product be used in conjugation with CS-C Medium for Endothelial Cell Lines (Product Nos. C 1431 or C 1556), Endothelial Attachment Factor (Product No. E 9765), Trypsin/EDTA Solution for Endothelial Cell Cultures (Product No. T 4299) and Trypsin Inhibitor Solution (Product No. T 6414).

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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C1556 CS-C Medium Without Serum for Endothelial Cell Lines, With L-glutamine, sodium bicarbonate, and HEPES, without phenol red, liquid, sterile-filtered, suitable for cell culture
C1431 CS-C Medium With Serum for Endothelial Cell Lines, With 10% iron supplemented bovine calf serum, L-glutamine, sodium bicarbonate, and HEPES, without phenol red, liquid, sterile-filtered, suitable for cell culture

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