E9644 Sigma


EGF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym: EGF, Epidermal Growth Factor human



Frequently Asked Questions

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Epidermal Growth Factor (EGF) is a small mitogenic polypeptide present throughout a large number of tissues and body fluids in many mammalian species. EGF is a member of a growth factor family characterized by 6 conserved cysteine motifs that form three disulfide bonds. Human EGF is an identical molecule to ß-urogastrone, a molecule isolated on the basis of its ability to inhibit gastric acid secretion. EGF is structurally homologous to human transforming growth factor-a, and both exert their actions through EGF receptors.

Analysis Note

The biological activity of this product is measured in a proliferation assay using BALB/MK cells. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.

Biochem/physiol Actions

EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, vascular and corneal endothelial cells, bovine granulosa, HeLa cells, SV40-3T3 cells and mammary epithelial cells. Stimulation of ion fluxes, glucose transport, glycolysis, and synthesis of DNA, RNA and proteins are all cellular metabolic effects of EGF. EGF also plays a biological role in inhibition of gastric acid secretion, support of growth during fetal development and neuromodulation in the central nervous system.


This product should be stored at -20°C and will retain activity for two years. After reconstitution, it can be stored at 2-8°C for one month, or frozen in aliquots at -70°C or -20°C for longer periods.


0.2 mg in glass bottle

Preparation Note

This product was lyophilized from a 0.2 μm-filtered solution of phosphate buffered saline, at pH 7.4. It should be reconstituted by adding the contents of the vial to 1 mg/mL using 10 mM acetic acid. To dilute to lower concentrations, but no fewer than 10 μg/mL, an addition of 0.1% BSA or HSA will be needed. For use in proliferation assays, further dilute the sample with medium without albumin.


This product is used as a mitogen, triggering the cell to commence mitosis, in a variety of cell lines. In tissue cultures, EGF acts to reduce or eliminate the requirement for serum and can be used in conjunction with other media additives and hormone.

Price and Availability

Polymeric Drug Delivery Techniques Guide

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Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Epidermal Growth Factors (EGF) and NRG Family

The epidermal growth factor (EGF) family of growth factors is comprised of the normal mammalian gene products of EGF, amphiregulin, betacellulin, heparin-binding EGF (HB-EGF), and transforming growth...
Jennifer Fries
BioFiles 2009, 4.5, 5.
Keywords: Cancer, Growth factors, PAGE, Type

Fibroblast Growth Factor Family (FGF)

Fibroblast Growth Factors (FGFs) are potent regulators of cell proliferation, differentiation and function and are critically important in normal development, tissue maintenance and wound repair. FGF...
Jennifer Fries
BioFiles 2009, 4.5, 6.
Keywords: Angiogenesis, Cell proliferation, Gene expression, Growth factors, Support

Growth Factors in Stem Cell Biology

Growth factors are naturally occurring regulatory molecules, which bind to receptors on the cell surface. They stimulate cell and tissue function through influencing cell differentiation by changing ...
Jennifer Fries
BioFiles 2009, 4.5, 11.
Keywords: Cellular processes, Diseases, Growth factors, Stem cell biology, Therapeutic uses

Hematopoietic Cytokines

Numerous cytokines are involved in the regulation of hematopoiesis within a complex network of positive and negative regulators. Some cytokines have very narrow lineage specificities of their actions...
Jennifer Fries
BioFiles 2009, 4.5, 8.
Keywords: Applications, Clinical, Growth factors, Ligands

Peer-Reviewed Papers


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A 3D human neural cell culture system for modeling Alzheimer's disease. Kim YH, Choi SH, D'Avanzo C, et al. Nat. Protoc. 10(7), 985-1006, (2015)


A phosphorylation-dependent intramolecular interaction regulates the membrane association and activity of the tumor suppressor PTEN Rahdar, M., et al. Proc. Natl. Acad. Sci. U. S. A. 106, 480-5, (2009)


Ataxia Telangiectasia Mutated (ATM) Inhibition Transforms Human Mammary Gland Epithelial Cells Mandriota, S.J., et al. J. Biol. Chem. 285, 13092-106, (2010)


Direct positive regulation of PTEN by the p85 subunit of phosphatidylinositol 3-kinase Chagpar, R.B., et al. Proc. Natl. Acad. Sci. U. S. A. 107, 5471-6, (2010)


Epimorphic regeneration approach to tissue replacement in adult mammals Agrawal, V., et al. Proc. Natl. Acad. Sci. U. S. A. 107, 3351-5, (2010)


Modulation of HER3 Is a Marker of Dynamic Cell Signaling in Ovarian Cancer: Implications for Pertuzumab Sensitivity Nagumo, Y., et al. Mol. Cancer Res. 7, 1563-71, (2009)


Unraveling the disease pathogenesis behind lethal hydrolethalus syndrome revealed multiple changes in molecular and cellular level. Honkala H Pathogenetics 2(1), 2, (2009)


Intrastriatal GDNF gene transfer by inducible lentivirus vectors protects dopaminergic neurons in a rat model of parkinsonism. Chen SS, Yang C, Hao F, et al. Exp. Neurol. 261, 87-96, (2014)

Role of Fas/FasL in regulation of inflammation in vaginal tissue during HSV-2 infection. Krzyzowska M, Shestakov A, Eriksson K, et al. Cell Death Dis. 2, e132, (2011)


MiR-34a targeting of Notch ligand delta-like 1 impairs CD15+/CD133+ tumor-propagating cells and supports neural differentiation in medulloblastoma. de Antonellis P PLoS ONE 6(9), e24584, (2011)


Nuclear entry of hyperbranched polylysine nanoparticles into cochlear cells. Weikai Zhang et al Int. J. Nanomedicine 6, 535-46, (2011)


Analysis of conjunctival fibroblasts from a proband with Schnyder corneal dystrophy. Jodhbir S. Mehta et al Mol. Vis. 14, 1277-81, (2008)


Treponema denticola major outer sheath protein induces actin assembly at free barbed ends by a PIP2-dependent uncapping mechanism in fibroblasts. Visser MB PLoS ONE 6(8), e23736, (2011)


The presenilin-1 familial Alzheimer's disease mutation P117L decreases neuronal differentiation of embryonic murine neural progenitor cells. Eder-Colli L Brain Res. Bull. 80(4-5), 296-301, (2009)


Robust methods for purification of histones from cultured mammalian cells with the preservation of their native modifications. Rodriguez-Collazo, P., et al. Nucleic Acids Res. 37(11), e81, (2009)


Protein kinase A-mediated CREB phosphorylation is an oxidant-induced survival pathway in alveolar type II cells. Barlow CA, Kitiphongspattana K, Siddiqui N, et al. Apoptosis 13(5), 681-92, (2008)


Chimeric IgG4 PR3-ANCA induces selective inflammatory responses from neutrophils through engagement of Fcgamma receptors. Hussain A Immunology 128(2), 236-44, (2009)


The research on the immuno-modulatory defect of mesenchymal stem cell from Chronic Myeloid Leukemia patients. Zhu Xishan et al J. Exp. Clin. Cancer Res 30, 47, (2011)


A spatiotemporally defined in vitro microenvironment for controllable signal delivery and drug screening. Kuo CT, Liu HK, Huang GS, et al. Analyst 139(19), 4846-54, (2014)


Calcium-sensing receptor-mediated osteogenic and early-stage neurogenic differentiation in umbilical cord matrix mesenchymal stem cells from a large animal model. Martino NA, Reshkin SJ, Ciani E, et al. PLoS ONE 9(11), e111533, (2014)


NPM1 silencing reduces tumour growth and MAPK signalling in prostate cancer cells. Loubeau G, Boudra R, Maquaire S, et al. PLoS ONE 9(5), e96293, (2014)


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