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E9644 Sigma

hEGF

EGF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym: EGF, Epidermal Growth Factor human

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Description

Frequently Asked Questions

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Components

Epidermal Growth Factor (EGF) is a small mitogenic polypeptide present throughout a large number of tissues and body fluids in many mammalian species. EGF is a member of a growth factor family characterized by 6 conserved cysteine motifs that form three disulfide bonds. Human EGF is an identical molecule to ß-urogastrone, a molecule isolated on the basis of its ability to inhibit gastric acid secretion. EGF is structurally homologous to human transforming growth factor-a, and both exert their actions through EGF receptors.

Analysis Note

The biological activity of this product is measured in a proliferation assay using BALB/MK cells. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.

Biochem/physiol Actions

EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, vascular and corneal endothelial cells, bovine granulosa, HeLa cells, SV40-3T3 cells and mammary epithelial cells. Stimulation of ion fluxes, glucose transport, glycolysis, and synthesis of DNA, RNA and proteins are all cellular metabolic effects of EGF. EGF also plays a biological role in inhibition of gastric acid secretion, support of growth during fetal development and neuromodulation in the central nervous system.

Caution

This product should be stored at -20°C and will retain activity for two years. After reconstitution, it can be stored at 2-8°C for one month, or frozen in aliquots at -70°C or -20°C for longer periods.

Packaging

0.2 mg in glass bottle

Preparation Note

This product was lyophilized from a 0.2 μm-filtered solution of phosphate buffered saline, at pH 7.4. It should be reconstituted by adding the contents of the vial to 1 mg/mL using 10 mM acetic acid. To dilute to lower concentrations, but no fewer than 10 μg/mL, an addition of 0.1% BSA or HSA will be needed. For use in proliferation assays, further dilute the sample with medium without albumin.

Application

This product is used as a mitogen, triggering the cell to commence mitosis, in a variety of cell lines. In tissue cultures, EGF acts to reduce or eliminate the requirement for serum and can be used in conjunction with other media additives and hormone.

Price and Availability


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Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Epidermal Growth Factors (EGF) and NRG Family

The epidermal growth factor (EGF) family of growth factors is comprised of the normal mammalian gene products of EGF, amphiregulin, betacellulin, heparin-binding EGF (HB-EGF), and transforming growth...
Jennifer Fries
BioFiles 2009, 4.5, 5.
Keywords: Cancer, Growth factors, PAGE, Type

Fibroblast Growth Factor Family (FGF)

Fibroblast Growth Factors (FGFs) are potent regulators of cell proliferation, differentiation and function and are critically important in normal development, tissue maintenance and wound repair. FGF...
Jennifer Fries
BioFiles 2009, 4.5, 6.
Keywords: Angiogenesis, Cell proliferation, Gene expression, Growth factors, Support

Growth Factors in Stem Cell Biology

Growth factors are naturally occurring regulatory molecules, which bind to receptors on the cell surface. They stimulate cell and tissue function through influencing cell differentiation by changing ...
Jennifer Fries
BioFiles 2009, 4.5, 11.
Keywords: Cellular processes, Diseases, Growth factors, Stem cell biology, Therapeutic uses

Hematopoietic Cytokines

Numerous cytokines are involved in the regulation of hematopoiesis within a complex network of positive and negative regulators. Some cytokines have very narrow lineage specificities of their actions...
Jennifer Fries
BioFiles 2009, 4.5, 8.
Keywords: Applications, Clinical, Growth factors, Ligands

Peer-Reviewed Papers

References

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Ataxia Telangiectasia Mutated (ATM) Inhibition Transforms Human Mammary Gland Epithelial Cells Mandriota, S.J., et al. J. Biol. Chem. 285, 13092-106, (2010)

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Direct positive regulation of PTEN by the p85 subunit of phosphatidylinositol 3-kinase Chagpar, R.B., et al. Proc. Natl. Acad. Sci. U. S. A. 107, 5471-6, (2010)

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Epimorphic regeneration approach to tissue replacement in adult mammals Agrawal, V., et al. Proc. Natl. Acad. Sci. U. S. A. 107, 3351-5, (2010)

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Modulation of HER3 Is a Marker of Dynamic Cell Signaling in Ovarian Cancer: Implications for Pertuzumab Sensitivity Nagumo, Y., et al. Mol. Cancer Res. 7, 1563-71, (2009)

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DRR regulates AKT activation to drive brain cancer invasion. Dudley A, Sater M, Le PU, et al. Oncogene 33(41), 4952-60, (2014)

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Autophagy promotes resistance to photodynamic therapy-induced apoptosis selectively in colorectal cancer stem-like cells. Wei MF, Chen MW, Chen KC, et al. Autophagy 10(7), 1179-92, (2014)

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Differential effects of the extracellular microenvironment on human embryonic stem cell differentiation into keratinocytes and their subsequent replicative life span. Movahednia MM, Kidwai FK, Zou Y, et al. Tissue Eng. Part A 21(7-8), 1432-43, (2015)

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Integrated analysis identifies different metabolic signatures for tumor-initiating cells in a murine glioblastoma model. Saga I, Shibao S, Okubo J, et al. Neuro. Oncol. 16(8), 1048-56, (2014)

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Propionibacterium acnes activates the NLRP3 inflammasome in human sebocytes. Li ZJ, Choi DK, Sohn KC, et al. J. Invest. Dermatol. 134(11), 2747-56, (2014)

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Role of STAT5 and epigenetics in lactation-associated upregulation of multidrug transporter ABCG2 in the mammary gland. Wu AM, Yang M, Dalvi P, et al. Am. J. Physiol. Endocrinol. Metab. 307(7), E596-610, (2014)

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MicroRNA-296-5p (miR-296-5p) functions as a tumor suppressor in prostate cancer by directly targeting Pin1. Lee KH, Lin FC, Hsu TI, et al. Biochim. Biophys. Acta 1843(9), 2055-66, (2014)

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Identification and analysis of a SMAD3 cis-acting eQTL operating in primary osteoarthritis and in the aneurysms and osteoarthritis syndrome. Raine EV, Reynard LN, van de Laar IM, et al. Osteoarthr. Cartil. 22(5), 698-705, (2014)

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Reconstituted human upper airway epithelium as 3-d in vitro model for nasal polyposis. de Borja Callejas F, Martínez-Antón A, Alobid I, et al. PLoS ONE 9(6), e100537, (2014)

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Two-photon imaging of remyelination of spinal cord axons by engrafted neural precursor cells in a viral model of multiple sclerosis. Greenberg ML, Weinger JG, Matheu MP, et al. Proc. Natl. Acad. Sci. U. S. A. 111(22), E2349-55, (2014)

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PI3K and Bcl-2 inhibition primes glioblastoma cells to apoptosis through downregulation of Mcl-1 and Phospho-BAD. Pareja F, Macleod D, Shu C, et al. Mol. Cancer Res. 12(7), 987-1001, (2014)

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Novel roles of focal adhesion kinase in cytoplasmic entry and replication of influenza A viruses. Elbahesh H, Cline T, Baranovich T, et al. J. Virol. 88(12), 6714-28, (2014)

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Targeting cancer stem-like cells as an approach to defeating cellular heterogeneity in Ewing sarcoma. Cornaz-Buros S, Riggi N, DeVito C, et al. Cancer Res. 74(22), 6610-22, (2014)

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Regulation of human growth hormone receptor expression by microRNAs. Elzein S, Goodyer CG, et al. Mol. Endocrinol. 28(9), 1448-59, (2014)

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A novel berbamine derivative inhibits cell viability and induces apoptosis in cancer stem-like cells of human glioblastoma, via up-regulation of miRNA-4284 and JNK/AP-1 signaling. Yang F, Nam S, Brown CE, et al. PLoS ONE 9(4), e94443, (2014)

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Angiogenin interacts with the plasminogen activation system at the cell surface of breast cancer cells to regulate plasmin formation and cell migration. Dutta S, Bandyopadhyay C, Bottero V, et al. Mol. Oncol. 8(3), 483-507, (2014)

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NEDD4 ubiquitinates TRAF3 to promote CD40-mediated AKT activation. Fang DF, He K, Wang N, et al. Nat. Commun. 5, 4513, (2014)

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Nitric oxide synthase activity is critical for the preovulatory epidermal growth factor-like cascade induced by luteinizing hormone in bovine granulosa cells. Zamberlam G, Sahmi F, Price CA, et al. Free Radic. Biol. Med. 74, 237-44, (2014)

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Putative tumor suppressor gene SEL1L was downregulated by aberrantly upregulated hsa-mir-155 in human pancreatic ductal adenocarcinoma. Liu Q, Chen J, Wang J, et al. Mol. Carcinog. 53(9), 711-21, (2014)

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Knockdown of GALNT1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing EGFR signaling. Huang MJ, Hu RH, Chou CH, et al. Oncotarget 6(8), 5650-65, (2015)

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Systemic anticancer neural stem cells in combination with a cardiac glycoside for glioblastoma therapy. Teng J, Hejazi S, Badr CE, et al. Stem Cells 32(8), 2021-32, (2014)

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Induction of dental epithelial cell differentiation marker gene expression in non-odontogenic human keratinocytes by transfection with thymosin beta 4. Kiyoshima T, Fujiwara H, Nagata K, et al. Stem Cell Res. 12(1), 309-22, (2014)

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Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate. Chou ML, Burnouf T, Wang TJ, et al. PLoS ONE 9(6), e99145, (2014)

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Vitamin D prevents endothelial progenitor cell dysfunction induced by sera from women with preeclampsia or conditioned media from hypoxic placenta. Brodowski L, Burlakov J, Myerski AC, et al. PLoS ONE 9(6), e98527, (2014)

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Functional cross-talk between the cellular prion protein and the neural cell adhesion molecule is critical for neuronal differentiation of neural stem/precursor cells. Prodromidou K, Papastefanaki F, Sklaviadis T, et al. Stem Cells 32(6), 1674-87, (2014)

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Defects in calcium homeostasis and mitochondria can be reversed in Pompe disease. Lim JA, Li L, Kakhlon O, et al. Autophagy 11(2), 385-402, (2015)

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RSK2 regulates endocytosis of FGF receptor 1 by phosphorylation on serine 789. Nadratowska-Wesolowska B, Haugsten EM, Zakrzewska M, et al. Oncogene 33(40), 4823-36, (2014)

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Multiplexing spheroid volume, resazurin and acid phosphatase viability assays for high-throughput screening of tumour spheroids and stem cell neurospheres. Ivanov DP, Parker TL, Walker DA, et al. PLoS ONE 9(8), e103817, (2014)

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The roles of testicular c-kit positive cells in de novo morphogenesis of testis. Zhang M, Zhou H, Zheng C, et al. Sci. Rep. 4, 5936, (2014)

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Urotensin II inhibits doxorubicin-induced human umbilical vein endothelial cell death by modulating ATF expression and via the ERK and Akt pathway. Chen YL, Tsai YT, Lee CY, et al. PLoS ONE 9(9), e106812, (2014)

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Heat-stable molecule derived from Streptococcus cristatus induces APOBEC3 expression and inhibits HIV-1 replication. Wang Z, Luo Y, Shao Q, et al. PLoS ONE 9(8), e106078, (2014)

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Leptin promotes wound healing in the oral mucosa. Umeki H, Tokuyama R, Ide S, et al. PLoS ONE 9(7), e101984, (2014)

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STAT3 modulation to enhance motor neuron differentiation in human neural stem cells. Natarajan R, Singal V, Benes R, et al. PLoS ONE 9(6), e100405, (2014)

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SMAD3 augments FoxO3-induced MuRF-1 promoter activity in a DNA-binding-dependent manner. Bollinger LM, Witczak CA, Houmard JA, et al. Am. J. Physiol. Cell Physiol. 307(3), C278-87, (2014)

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Soluble-E-cadherin activates HER and IAP family members in HER2+ and TNBC human breast cancers. Brouxhon SM, Kyrkanides S, Teng X, et al. Mol. Carcinog. 53(11), 893-906, (2014)

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Up-regulated cten by FGF2 contributes to FGF2-mediated cell migration. Hung SY, Shih YP, Chen M, et al. Mol. Carcinog. 53(10), 787-92, (2014)

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Function and expression of ATIP and its variants in cardiomyoblast cell line H9c2. Varghayee N, Krezel MA, Rezmann L, et al. J. Renin Angiotensin Aldosterone Syst. 16(1), 79-91, (2015)

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Cancer stem-like sphere cells induced from de-differentiated hepatocellular carcinoma-derived cell lines possess the resistance to anti-cancer drugs. Hashimoto N, Tsunedomi R, Yoshimura K, et al. BMC Cancer 14, 722, (2014)

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Overexpression of cyclin dependent kinase inhibitor P27/Kip1 increases oligodendrocyte differentiation from induced pluripotent stem cells. Tamaki S, Tokumoto Y, et al. In Vitro Cell. Dev. Biol. Anim. 50(8), 778-85, (2014)

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Antineoplastic activity of the multitarget tyrosine kinase inhibitors CLM3 and CLM94 in medullary thyroid cancer in vitro. Ferrari SM, Fallahi P, La Motta C, et al. Surgery 156(5), 1167-76, (2014)

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miR-125b inhibitor enhance the chemosensitivity of glioblastoma stem cells to temozolomide by targeting Bak1. Chen J, Fu X, Wan Y, et al. Tumour Biol. 35(7), 6293-302, (2014)

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