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  • E9653 - Monoclonal Anti-Endothelial Cells antibody produced in mouse

E9653 Sigma

Monoclonal Anti-Endothelial Cells antibody produced in mouse

clone P1H12, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-CD146



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins, Antibodies to Cell and Organelle Proteins,
biological source   mouse
antibody form   purified immunoglobulin
clone   P1H12, monoclonal
form   buffered aqueous solution
mol wt   antigen mol wt 130 kDa
species reactivity   human
application(s)   flow cytometry: suitable using 2% paraformaldehyde 9:1 methanol
  immunocytochemistry: suitable using 4% paraformaldehyde 0.1-0.5% triton X-100, smears or live cells
  immunohistochemistry: suitable using using acetone-fixed sections of human tongue
  immunoprecipitation: suitable
  indirect ELISA: suitable
  microarray: suitable
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C


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human umbilical cord cells (HUVEC).


Recognizes cultured microvascular and large-vessel endothelial cells (MVEC and HUVEC, respectively) and endothelial cells in tissues and in circulation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide


Monoclonal Anti-Endothelial Cells antibody produced in mouse is suitable for the following applications:
• Flow cytometry using 2% paraformaldehyde 9:1 methanol
• Immunocytochemistry (using 4% paraformaldehyde 0.1-0.5% triton X-100, smears or live cells) Immunohistochemistry (using using acetone-fixed sections of human tongue)
• Immunoprecipitation
• Indirect ELISA
• Microarray

General description

CD146 (cluster of differentiation 146) is also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18. It is encoded by the MCAM gene. Soluble CD146 is associated with the activation of angiogenesis. Circulating endothelial cells (CECs) may help in predicting clinical outcome in cancer patients. Tumor endothelial markers are expressed in a wide range of tumor types and in normal vessels associated with wound healing and corpus luteum formation. It may have significant implications for the development of anti-angiogenic therapies as well.

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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