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E9762 Sigma

Endoglycosidase F1 from Elizabethkingia miricola

recombinant, expressed in E. coli, ≥16 U/mg, buffered aqueous solution

Synonym: Endo F1, Endo-β-N-acetylglucosaminidase F1, Endoglycosidase F1 from Chryseobacterium meningosepticum, Endoglycosidase F1 from Elizabethkingia meningoseptica, Endoglycosidase F1 from Flavobacterium meningosepticum

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Description

Application

Cleaves asparagine-linked or free oligomannose and hybrid, but not complex, oligosaccharides.

Packaging

Supplied with 5× Reaction Buffer, 250 mM NaH2PO4, pH 5.5.

Unit Definition

One unit will release N-linked oligosaccharides from 1 μmole of denatured Ribonuclease B in 1 minute at 37 °C, pH 5.5.

Physical form

Aseptically filled solution in 20 mM Tris-HCl pH 7.5

Price and Availability


Amplified Detection

Amicon Pro Purification System
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Endoglycosidases

For some glycoproteins, no cleavage by PNGase F occurs unless the protein is denatured. For others, some or all of the oligosaccharides can be removed from the native protein after extensive incubati...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Buffers, Fucosylation, Type

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Peer-Reviewed Papers
15

References

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