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E9765 Sigma

Endothelial Cell Attachment Factor (ECAF)

liquid, sterile-filtered, suitable for cell culture

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Properties

Related Categories Cell Culture, Endothelial Cells, Human Umbilical Vein Endothelial Cell (HUVEC) Culture Companion Products and Supplements, Primary Human Cell Culture, Specialty Media Systems More...
sterility   sterile-filtered
form   liquid
impurities   endotoxin, tested
suitability   suitable for cell culture
shipped in   dry ice
storage temp.   −70°C

Description

Formula variant

With gelatin, factors from iron-supplemented calf serum associated with endothelial cell attachment and spreading, sodium bicarbonate and HEPES buffers, and phenol red.

Application

ECAF is optimized to promote the attachment, spreading and polarity of endothelial cells. It contains gelatin (in HEPES buffered medium) to which factors separated from serum by chromatography and dialysis have been added. Use in combination with C1431, C1556, E9640 and T4299.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Related Products

recommended

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Description

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T4299 Trypsin-EDTA solution, 1 ×, sterile-filtered, BioReagent, suitable for cell culture, 500 BAEE units porcine trypsin and 180 μg EDTA • 4Na per ml in Dulbecco′s PBS without calcium and magnesium
C1556 CS-C Medium Without Serum for Endothelial Cell Lines, With L-glutamine, sodium bicarbonate, and HEPES, without phenol red, liquid, sterile-filtered, suitable for cell culture
E9640 Endothelial cell growth factor, 100 ×, sterile-filtered, suitable for cell culture
C1431 CS-C Medium With Serum for Endothelial Cell Lines, With 10% iron supplemented bovine calf serum, L-glutamine, sodium bicarbonate, and HEPES, without phenol red, liquid, sterile-filtered, suitable for cell culture

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