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  • E9906 - Anti-EDEM2 (N-terminal) antibody produced in rabbit

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E9906 Sigma

Anti-EDEM2 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-ER degradation enhancer, mannosidase alpha-like 2

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to Oxidative Stress Proteins,
species reactivity   mouse, human, rat (predicted)
application(s)   indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells
  western blot: 0.5-1 μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM2
clone   polyclonal
concentration   ~1.0 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~70 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... EDEM2(55741)
mouse ... Edem2(108687)
rat ... Edem2(296304)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 22-38 of human EDEM2, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and 2 amino acids in rat EDEM2.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-EDEM2 (N-terminal) antibody produced in rabbit is suitable for indirect immunofluorescence at a concentration of 5-10μg/mL using mouse 3T3 cells and western blotting at a concentration of 0.5-1μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM2.

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Biochem/physiol Actions

ER degradation-enhancing α-mannosidase-like 2 is an enzyme encoded by the EDEM2 gene in humans. It is one of the ER-stress-induced members of the glycosyl hydrolase 47 family. EDEM2 is a novel, stress-regulated mannosidase-like protein that operates in the ER lumen. The transcriptional up-regulation of EDEM2 depends on the ER stress-activated transcription factor Xbp1 that accelerates ER-associated degradation (ERAD) of terminally misfolded glycoproteins by facilitating their extraction from the calnexin cycle.

Price and Availability


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