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Enzymatic Protein Deglycosylation Kit




Contains all enzymes and reagents needed to completely remove all N- and simple O-linked carbohydrates from glycoproteins, including polysialylated glycans, as well as cleavage of complex Core 2 O-linked carbohydrates including those containing polylactosamine.

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Amplified Detection

Biomedical Applications

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PNGase F 1 mL/vial SDS    
O-Glycosidase 20 μL SDS    
α-2(3,6,8,9)-Neuraminidase 20 μL SDS    
Fetuin Control .5 mg SDS    
5× Reaction Buffer .2 mL SDS    
Denaturation Solution .1 mL SDS    
Triton X-100 .1 mL SDS    
β-1→4-Galactosidase, positionally specific 20 μL SDS    
β-N-Acetylglucosaminidase 20 μL SDS    
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Protocols & Articles


Deglycosylation Kits

GlycoProfile™ I Enzymatic In-gel N-Deglycosylation Kit GlycoProfile™ II Enzymatic In-solution N-Deglycosylation Kit Enzymatic Protein Deglycosylation Kit Native Protein Deglycosylation Kit
Glycobiology Analysis Manual, 2nd Edition
Keywords: Degradations, Digestions, Glycosylations, High performance liquid chromatography, Mass spectrometry, Methods, PAGE, Proteomics

GlycoProfile™ Labeling Kits: Useful Fluorescent Dyes for Enhanced Glycan Analysis

Glycan analysis has become an increasingly critical aspect of glycomics and proteomics, as the role of glycoproteins in cell signaling, cell adhesion, immune response, and disease states is emerging ...
BioFiles 2007, 2.1, 14.
Keywords: Adhesion, Aminations, Atomic absorption spectroscopy, Capillary electrophoresis, Cell signaling, Chromatography, Coupling reactions, Filtration, Glycosylations, High performance liquid chromatography, Ion Exchange, Mass spectrometry, Methods, Normal-phase chromatography, Phase transitions, Proteomics, Reductions, Reductive aminations, Separation, Tools

Glycoprotein Deglycosylation

Aparagine-linked (N-linked) and serine/threonine-linked (O-linked) oligosaccharides are major structural components of many eukaryotic proteins. They perform critical biological functions in protein ...
BioFiles 2007, 2.1, 7.
Keywords: Degradations, Dialysis, Digestions, Filtration, Glycosylations, High performance liquid chromatography, Inflammation, Ligands, Mass spectrometry, Methods, PAGE, Proteomics, Substitutions, Type

N-Linked Glycan Strategies

Use of the endoglycosidic enzyme PNGase F (N-Glycosidase F) is the most effective method of removing virtually all N-linked oligosaccharides from glycoproteins. PNGase F cleaves all asparagine-linked...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Applications, Detergents, Digestions, Mass spectrometry, Methods, Nucleic acid denaturation, Proteomics

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