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EXTRA3 Sigma

ExtrAvidin® Peroxidase Staining Kit for rabbit antibodies antibody produced in mouse

Synonym: ExtrAvidin® Peroxidase Staining Kits

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Description

Components

• 3 mL ExtrAvidin®-Peroxidase
• 3 mL Biotinylated Secondary Antibodies
• Complete Instructions and Assay Protocols

Features and Benefits

• Use in immunohistology, ELISA, and immunoblotting assays.
• Affinity Isolated Antibodies have been adsorbed with human IgG and IgM to minimize cross-reactivity.
• Biotinylated antibodies contain a spacer which improves accessibility for the ExtrAvidin® conjugates.

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

General description

These kits comprise universal reagents for use with primary antibodies in immunohistology, ELISA, and immunoblotting.
ExtrAvidin® is a unique form of avidin, available only from Sigma, that combines the high specificity and affinity of avidin for biotin with low non-specific binding at physiological pH. ExtrAvidin® peroxidase exhibits high sensitivity with low background.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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