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F0291 Sigma

hBFGF

FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture

Synonym: FGF2, Fibroblast Growth Factor-Basic human, bFGF

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Description

Analysis Note

The bioactivity of FGF-Basic was measured in a fluorometric assay using the redox sensitive dye, Resazurin.

General description

Fibroblast growth factor-basic (FGF-Basic) is found in basement membranes and sub-endothelial extracellular matrix. FGF-Basic is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF-Basic is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF-Basic in human ES cells.

Physical form

Lyophilized from 0.2 μm filtered solution in 20 mM Tris and 1 M NaCl, pH 7.0, containing 50 μg bovine serum albumin per 1 μg BFGF.

Application

Fibroblast growth factor-basic (FGF-Basic) is required for the maintenance of human embryonic stem cells in culture, and various other stem cell lines, such as mesenchymal stem (stroma) cells (MSC).

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
LK3641325

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Mesenchymal stem cells (MSCs) have the capacity for multi-lineage differentiation, giving rise to a variety of mesenchymal phenotypes such as osteoblasts (bone), adipocytes (fat), and chondrocytes (c...
Anna Abai, Nick Asbrock and Vi Chu1
1 MilliporeSigma, Bioscience Division, Temecula, CA, USA
 
Keywords: Cell culture, Clinical, Drug discovery, Gene expression, Immunostaining

Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

Introduction Methods    Human Neural Stem Cell Culture    Oligodendrocyte Differentiation and Maturation    Oligodendrocyte Progenitor Cell Characterization    In Vitro Myelination Assay Results Conc...
Christine Chen, Michael Moeller, Anna Abai, Nick Asbrock and Vi Chu,1
1
MilliporeSigma, Bioscience Division, Temecula, CA, USA
 
Keywords: Adhesion, Atomic absorption spectroscopy, Cell culture, Cell proliferation, Central Nervous System, Clinical, Culture media, Diseases, Gene expression, Growth factors, Hormones, Neurodegenerative Diseases, Pharmaceutical, Phase transitions, RNA immunoprecipitation, Transfection

Fibroblast Growth Factor Family (FGF)

Fibroblast Growth Factors (FGFs) are potent regulators of cell proliferation, differentiation and function and are critically important in normal development, tissue maintenance, wound repair and ang...
Keywords: Angiogenesis, Cancer, Cardiovascular, Cell proliferation, Diabetes, Diseases, Gene expression, Growth factors, Ligands

Growth Factors in Stem Cell Biology

Growth factors are naturally occurring regulatory molecules, which bind to receptors on the cell surface. They stimulate cell and tissue function through influencing cell differentiation by changing ...
Keywords: Adhesion, Angiogenesis, Cell biology, Cell proliferation, Cellular processes, Diseases, Growth factors, Hormones, Stem cell biology, Therapeutic uses, Transcription, Transfection

Naive Pluripotent Stem Cell Culture in an Inhibitor Based 2i/LIF Containing Serum-Free and Feeder-Free Stem Cell Media

Naive pluripotent stem cells are located within the epiblast of mature blastocysts. These primitive “ground-state” cells may be cultured in vitro using specialized media and small molecule inhibitors...
Gabi Tremml, Ming Li and Vi Chu1*,
1MilliporeSigma, Bioscience Division, Temecula, CA, USA
 
Keywords: Cell culture, Gene expression, Immunocytochemistry, Microscopy, Polymerase chain reaction, Polymerase chain reaction - quantitative, Transcription

Neural Stem Cell FAQs

The use of neural stem cells (NSCs) in biomedical research is becoming increasingly popular, resulting in breakthrough studies rejected the longstanding belief that neuronal tissue is incapable of re...
Keywords: Cell culture, Central Nervous System, Centrifugation, Clinical, Diffusion, Digestions, Diseases, Gene expression, Immunocytochemistry, Neurodegenerative Diseases, Parkinson Disease

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

Introduction Methods       Human MSC Expansion in Human Platelet Lysate       Human MSC Differentiation       FACS Mesenchymal Stem Cell Marker Analysis Results Conclusions Materials
Aletta Schnitzler, Manjula Aysola, Anjali Verma, Tristan Lawson, Susan Rigby, Megan Pease, Sandhya Punreddy, Julie Murrell, Martha Rook1

1 EMD Millipore Corporation, Stem Cell Bioprocessing Group, 80 Ashby Rd, Bedford, MA 01730, USA.
Keywords: Cell culture, Cell disruption, Clinical, Culture media, Flow cytometry, Gene expression, Growth factors, Polymerase chain reaction, Stem cell biology

Predictive Models for Neuroscience Research

For neuroscientists, the quest for truly predictive neural models has its challenges given the brain is a very complex organ comprised of many diverse cell types, complex cell-tocell interactions and...
Keywords: Apoptosis, Cell culture, Cell proliferation, Central Nervous System, Deposition, Diseases, Dopamine agents, Drug discovery, Environmental, Filtration, Gene expression, Genetic, Growth factors, Neuroscience, Reductions, Schizophrenia, Sequencing, Sterilizations, Transfection

Protocols

Mesenchymal Stem Cell Culture Protocols

Introduction Methods   Isolation of Mesenchymal Stem Cells   Expansion of Human Mesenchymal Stem Cells     Preparation of Coated Plates     Thawing of Mesenchymal Stem Cells     Expansion of Mesenchy...
Keywords: Cell disruption, Clinical, Culture media, Gene expression, Immunocytochemistry, Stem cell biology

Neural Stem Cell Culture Protocols

Introduction Methods     Isolation of Neural Stem Cells         Isolation of NSCs from Neural Tissue         Differentiation of Human iPSCs into NSCs         Characterization of Neural Stem Cells    ...
Keywords: AGE, Adhesion, Apoptosis, Cell culture, Central Nervous System, Cryopreservation, Culture media, Diseases, Growth factors, High performance liquid chromatography, Phase transitions, Phosphorylations, Purification

Peer-Reviewed Papers
15

References

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