|Related Categories||Angiogenesis, Angiogenic Factors, Cancer Research, Cell Biology, Cell Culture,|
|recombinant||expressed in E. coli|
|suitability||suitable for cell culture|
|Gene Information||human ... FGF12(2257)|
The bioactivity of FGF-Basic was measured in a fluorometric assay using the redox sensitive dye, Resazurin.
FGF-Basic is required for the maintenance of human embryonic stem cells in culture, and various other stem cell lines such as mesenchymal stem (stroma) cells (MSC).
Fibroblast growth factor-basic (FGF-Basic) is found in basement membranes and sub-endothelial extracellular matrix. FGF-Basic is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF-Basic is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF-Basic in human ES cells.
Lyophilized from 0.2 μm filtered solution in 20 mM Tris and 1 M NaCl, pH 7.0, containing 50 μg bovine serum albumin per 1 μg BFGF.
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FGF-Basic, suitable for cell culture
FGF-Basic, recombinant, expressed in E. coli, sterile-filtered, aqueous solution, suitable for cell culture
Fibroblast Growth Factors (FGFs) are potent regulators of cell proliferation, differentiation and function and are critically important in normal development, tissue maintenance and wound repair. FGF...
BioFiles 2009, 4.5, 6.
Keywords: Angiogenesis, Cell proliferation, Gene expression, Growth factors
Fibroblast growth factor 2 modulates transforming growth factor-β signaling in mouse embryonic fibroblasts and human ESCs (hESCs) to support hESC self-renewal Greber B, et al. Stem Cells 25, 455-464, (2007)
Complete Focal Adhesion Kinase Deficiency In The Mammary Gland Causes Ductal Dilation And Aberrant Branching Morphogenesis Through Defects In Rho Kinase-dependent Cell Contractility. van Miltenburg, M.H., et al. FASEB J. 23, 3482-93, (2009)
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|F5180||Anti-Fibroblast Growth Factor-Basic antibody produced in goat, affinity isolated antibody, lyophilized powder|
|F5305||Monoclonal Anti-Fibroblast Growth Factor-Basic antibody produced in mouse, clone 10060, purified immunoglobulin, lyophilized powder|
|F6162||Monoclonal Anti-Fibroblast Growth Factor-Basic antibody produced in mouse, clone FB-8, purified immunoglobulin, buffered aqueous solution|
|F5537||Anti-Fibroblast Growth Factor-Basic antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution|
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