Fibroblast Growth Factor-Basic human
recombinant, expressed in E. coli, suitable for cell culture
DOWNLOAD MSDS (PDF)Synonym: FGF2, bFGF
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CAS Number 106096-93-9
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MDL number MFCD00081382
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Popular Documents: Datasheet (PDF) | Specification Sheet (PDF)
Properties
| Related Categories | Angiogenesis, Angiogenic Factors, Cancer Research, Cell Biology, Cell Culture, |
| recombinant | expressed in E. coli |
| assay | ≥97% (SDS-PAGE) |
| form | lyophilized powder |
| suitability | suitable for cell culture |
| storage temp. | −20°C |
| Gene Information | human ... FGF12(2257) |
Description
Analysis Note
The bioactivity of bFGF was measured in a fluorometric assay using the redox sensitive dye, Resazurin.
Application
FGF-2 is required for the maintenance of human embryonic stem cells in culture, and various other stem cell lines such as mesenchymal stem (stroma) cells (MSC).
General description
FGF2 is found in basement membranes and sub-endothelial extracellular matrix. FGF2 is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF2 is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF2 in human ES cells.
Physical form
Lyophilized from 0.2 μm filtered solution in 20 mM Tris and 1 M NaCl, pH 7.0, containing 50 μg bovine serum albumin per 1 μg BFGF.
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