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F0425 Sigma

Anti-Fibroblast Growth Factor Receptor-3, Cytoplasmic antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-FGFR-3

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Cytokines, Chemokines, and Growth Factors for Stem Cell Biology, Antibodies against Cytokines/Growth Factors/Chemokines for Stem Cell Biology, Antibodies for Cell Biology,
species reactivity   human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:250 using Trypsin-digested, human and animal tissue sections
  western blot: 1:1,000 using whole cell extract of transfected 293T cells expressing recombinant human FGFR-3
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 110-120 kDa (doublet)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FGFR3(2261)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 792-806 of the cytoplasmic region of human FGFR-3 with N-terminal added lysine.

General description

Fibroblast Growth Factor Receptor-3 (FGFR 3) is a protein that belongs to Tyr protein kinase family and is expressed in various fetal and adult human and animal tissues. It plays a vital role in inducing apoptosis in chondrogenic ATDC5 cells. It also facilitates cell proliferation and differentiation. Anti-Fibroblast Growth Factor Receptor-3, cytoplasmic antibody can be used for western blotting (diluted 1:1,000) using whole cell extracts of transfected 293T cells expressing recombinant human FGFR-3. Rabbit anti-Fibroblast Growth Factor Receptor-3, cytoplasmic antibody reacts specifically with human FGFR-3 but not with human FGFR-1 or FGFR-2.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

The antibody reacts specifically with FGFR-3 in lysates of transfected cells. No reaction with FGFR-1 and FGFR-2 is detected.

Application

Anti-Fibroblast Growth Factor Receptor-3, cytoplasmic antibody can be used in immunohistochemistry (diluted 1:250) using Trypsin-digested, human and animal tissue sections for identification of FGFR-3. It can also be used in immunoprecipitation.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  2
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Peer-Reviewed Papers
15

References

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