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F0522 Sigma

Monoclonal Anti-CD3−FITC antibody produced in mouse

clone UCHT-1, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-CD3

Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood,
species reactivity   human
application(s)   flow cytometry: 10 μL using 1 × 106 cells
clone   UCHT-1, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... CD3E(916)
biological source   mouse
conjugate   FITC conjugate

Description

Immunogen

human thymocytes followed by Sezary T cells.

Antigen Background

The CD3 complex (16/20/29/22/25-28 kDa) consists of 5 polypeptide chains associated with each other and with the T cell receptor.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

Recognizes the CD3 complex. The epitope recognized by clone no. UCHT-1 is expressed on the ε-chain of the CD3 antigen/T cell receptor complex. 3rd Workshop: code no. 126, 208 and 471
4th Workshop: paper no. T3.2

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Application

Monoclonal Anti-CD3-FITC antibody produced in mouse is suitable for flow cytometry (at a dilution of 10μL using 1 × 106 cells). It was also used for ADCC Assay.

Features and Benefits

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Biochem/physiol Actions

CD3e molecule, ε (CD3E) is a polypeptide encoded by the CD3E gene in humans. It resides on chromosome 11. The CD3ε cytoplasmic domain of the T cell receptor binds to the inner leaflet of the plasma membrane (PM) and is accessible during early stages of T cell activation. It is specific for T lymphocytes and is found in Purkinje cells of the cerebellum as well as in gastric parietal cells of humans and other species. The antibodies commonly used to recognize CD3 are directed against the ε-subunit of the T cell receptor. Cytoplasmic cyCD3 expression can only be seen during the early phase of NK-cell differentiation.

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