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F0652 Sigma

Anti-Factor IX antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood, Antibodies to Clotting Factors,
species reactivity   human
application(s)   dot blot: 1:8,000
  indirect ELISA: 1:5,000
  western blot: 1:3,000 using using reduced and non-reduced human plasma blots
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... F9(2158)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

human factor IX

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Specificity

Specifically reacts with human factor IX. The antibody detects human factor IX in both reduced and non-reduced normal human plasma.

Application

Anti-Factor IX antibody produced in rabbit is suitable for the following applications:
• Dot blot analysis at a dilution of 1:8,000
• Indirect ELISA at a dilution of 1:5,000
• Western blotting at a dilution of 1:3,000 using using reduced and non-reduced human plasma blots

Features and Benefits

Antibody Bioguarantee
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Biochem/physiol Actions

Four and a half LIM domains protein 1 is a protein encoded by the FHL1 gene in humans and is located on human chromosome Xq27.2. Factor IX (or Christmas factor) is one of the serine proteases of the coagulation system. It belongs to peptidase family S1 and its deficiency causes hemophilia B. The proteins belong to a novel family of LIM proteins that are expressed in human skeletal muscle. FHL1 gene is related to carcinogenesis and its inactivation is a frequent event during oral carcinogenesis. FHL1 downregulation in oral squamous cell carcinoma (OSCC) occurs through DNA methylation of the promoter region rather than histone deacetylation or mutation.

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