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F0895 Sigma

Fibronectin from human plasma

liquid, 0.1% (Solution), BioReagent, suitable for cell culture

Synonym: CIG, Cold insoluble globulin

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

Fibronectin is an adhesive glycoproteins found in both the cell surface and in plasma.

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Application

Fibronectin from human plasma can be used with epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Preparation Note

This product is a 0.1% solution of fibronectin. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.

In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C

Other Notes

Insolubles in our lyophilized fibronectin products is normal. Adding warm water carefully to the vial will, over time, provide a clear solution under a pellicle, a floating spider web-like material. Vials are filled with sufficient additional material to compensate for the loss of fibronectin in the pellicle. Protein concentration is tested in the reconstituted solution to ensure that the vials contain a minimum of the stated protein in a soluble form. Shaking or vortexing fibronectin in a liquid form will further denature the protein leading to decreased solubility.

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Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How should I store and handle Product F0895, Fibronectin from human plasma?
Recommended storage of this material is  2-8 °C.  Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.
Is Product F0895, Fibronectin from human plasma, sterile?
Fibronectin 0.1% solution is a sterile product.  It is tested for sterility.  The sterility test results can be found on the lot-specific certificate of analysis.
What is the solvent for Product F0895, Fibronectin from human plasma?
This material is solubilized in a 0.05 M Tris-buffered saline, pH 7.5.
How much Product F0895, Fibronectin from human plasma, do I use for my application?
Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
Show more questions
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Fibronectin (FN)

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Fibronectin Cell Attachment Protocol

Freezing and thawing of reconstituted fibronectin is not recommended as breakdown of protein will occur.
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Peer-Reviewed Papers
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References

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