• USA Home
  • F0895 - Fibronectin from human plasma

EMAIL THIS PAGE TO A FRIEND
F0895 Sigma

Fibronectin from human plasma

liquid, 0.1% (Solution), BioReagent, suitable for cell culture

Synonym: CIG, Cold insoluble globulin

Purchase

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

Fibronectin is an adhesive glycoproteins found in both the cell surface and in plasma.

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Application

Fibronectin from human plasma can be used with epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Preparation Note

This product is a 0.1% solution of fibronectin. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.

In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C

Other Notes

Insolubles in our lyophilized fibronectin products is normal. Adding warm water carefully to the vial will, over time, provide a clear solution under a pellicle, a floating spider web-like material. Vials are filled with sufficient additional material to compensate for the loss of fibronectin in the pellicle. Protein concentration is tested in the reconstituted solution to ensure that the vials contain a minimum of the stated protein in a soluble form. Shaking or vortexing fibronectin in a liquid form will further denature the protein leading to decreased solubility.

Price and Availability


Filter out uncertainty.

Discover the Milli-Q® IQ 7000 ultrapure water system
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Fibronectin Cell Attachment Protocol

Freezing and thawing of reconstituted fibronectin is not recommended as breakdown of protein will occur.
George Sitterley
BioFiles 2008, 3.8, 9.
Keywords: Phase transitions

Fibronectin

Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein composed of two nearly identical disulfidebound polypeptides of molecular weight 220 kDa. Cellular fibronectin is structurally...
George Sitterley
BioFiles 2008, 3.8, 8.
Keywords: Adhesion, Cell culture, transformation

The Cancer Stem Cell Hypothesis

Traditionally, cancer has been viewed as a disease in which environmental or endogenous events induce mutations to critical oncogenes and tumor suppressor genes within a normal cell. The clinical man...
Keywords: Apoptosis, Asymmetric synthesis, Cancer, Catalysis, Cell culture, Cell division, Cell proliferation, Cell signaling, Clinical, Degradations, Environmental, Gastrointestinal, Gene expression, Ligands, Phosphorylations, Poisons, Transcription, Transfection, transformation

Related Content

癌症干细胞

传统观点认为,癌症是环境或内部因素诱导正常细胞内的关键致癌基因和肿瘤抑制基因突变导致的一种疾病。当这些突变导致细胞向更原始、高度增殖状态转化,克隆扩张形成白血病或实体肿瘤时,就会出现癌症的临床表现1。然而,这种模式不能够完全解释许多肿瘤和转移瘤的长发育延迟,产生细胞去分化和细胞永生的起始诱导机制,或肿瘤本身内的细胞功能和表型多样性起源。过去十年中,越来越多的证据表明,恶性肿瘤起源于组织干细胞在突...
Keywords: Angiogenesis, Cancer, Eliminations, Gene expression, Growth factors, Ligands, Metabolic Pathways, Phosphorylations

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?