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F0916 Sigma

Monoclonal Anti-Fibronectin antibody produced in mouse

clone IST-4, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Stem Cell Biology,
species reactivity   human
does not react with   mouse, canine, chicken, rabbit, goat, bovine
application(s)   immunocytochemistry: 1:100 using cultured human fibroblasts
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunohistochemistry (frozen sections): suitable
  indirect ELISA: suitable
  radioimmunoassay: suitable
  western blot: 1:250 using human fibroblasts cell extract
clone   IST-4, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 240 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FN1(2335)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human plasma fibronectin

General description

Fibronectin (FN) is an extracellular matrix protein composed of two nearly-identical disulfide-bound polypeptides with typical molecular weights of 220-280 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma and antibodies to either form usually cross-react. Careful analysis of the FN molecule indicates that it contains several functionally and structurally distinct domains which may bind to cell surfaces and to a variety of molecules such as collagen, heparin, gelatin, fibrin and DNA. FN’s play an important role in diverse biological phenomena including cell adhesion, cell migration, hemostasis and thrombosis, wound healing and the ability to induce a more normal phenotype in transformed cells.

Mouse monoclonal clone IST-4 anti-Human Fibronectin antibody recognizes an epitope located within the 5th type III repeat of human plasma fibronectin. This epitope is common to all fibronectin forms. The antibody localizes plasma and cellular fibronectin both in its native and denatured-reduced forms (240 kDa) applying immunoblotting, RIA, ELISA, immunocytochemistry and immunohistochemistry. The product reacts with human fibronectin. No reactivity is observed with other species including goat, bovine, dog, rabbit, mouse and chicken.

Application

Mouse monoclonal clone IST-4 anti-Human Fibronectin antibody may be used for the specific labeling of fibronectin in cultured cells and in frozen tissue sections. Staining of formalin-fixed, paraffin-embedded sections may require prolonged proteolytic unmasking (e.g., by pepsin but not by trypsin).

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