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F1043 Sigma

Monoclonal Anti-Filensin antibody produced in mouse

clone FIL-7B10, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
species reactivity   human, sheep, bovine
does not react with   mouse, chicken, rat
application(s)   immunohistochemistry (frozen sections): suitable
  indirect immunofluorescence: 1:400 using bovine lens frozen sections
  microarray: suitable
  western blot: suitable
clone   FIL-7B10, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 115 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... BFSP1(631)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human and bovine lens filament enriched fraction (plasma membrane-cytoskeleton complex).

Specificity

The antibody recognizes an epitope located within either the rod or the N-terminal non-(α) helical domain of the filensin molecule. May be used in immunoblotting where it specifically detects both the full-length filensin (115 kDa), the processed fragment, and a variety of breakdown products in the mol. wt. range of 28-69 kDa. Does not react with cultured lens epithelial cells nor with normal human skin, psoriatic or tumoral skin (basal cell carcinoma).

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