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F137 Sigma

Anti-c-Fos antibody produced in rabbit

IgG fraction of antiserum, lyophilized powder

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Gene Regulation, Antibodies to Common Transcription Factors, Antibodies to Transcription Factors,
species reactivity   mouse
application(s)   western blot (chemiluminescent): 10 μg/mL using mouse NIH3T3 cells
clone   polyclonal
antibody form   IgG fraction of antiserum
form   lyophilized powder
mol wt   antigen mol wt 50-60 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FOS(2353)
mouse ... Fos(14281)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide derived from amino acids 4-17 from the N-terminal region of human and mouse c-Fos.

Physical form

Lyophilized from phosphate buffered saline containing 0.05% sodium azide.

Specificity

The antibody does not react with c-Fos in HeLa cells.

Preparation Note

Prepared using protein A.

Application

Anti-c-Fos antibody produced in rabbit is suitable for the following applications:
• Western blotting (chemiluminescent) at a concentration of 10μg/mL using mouse NIH3T3 cells1
• Immunohistochemistry2,3,4
• Tissue Immunofluorescence5

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Reconstitution

Reconstitute with 100 μl deionized water.

Biochem/physiol Actions

c-Fos is a proto-oncogene, which is the human homolog of the retroviral oncogene v-fos. It is one of the essential transcription factors that helps in initiation of adipocyte differentiation.6 FOS protein expression in human glioma is found to be strong and probably plays a critical role in the progression of gliomas.7 c-Fos may be involved in regulating lipid synthesis.8 c-Fos is associated with prognosis of ovarian cancer (OvCa) by changing the adhesion of OvCa cells to peritoneal surfaces.9

Price and Availability

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