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F1418 Sigma

Ficoll® 400

BioXtra, Type 400-DL, lyophilized powder

Synonym: Polysucrose 400

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Properties

Related Categories Biochemicals and Reagents, Carbohydrates, Carbohydrates A to Z, Carbohydrates D-F, Carbohydrates for Protein Stabilization,
type   Type 400-DL
product line   BioXtra
form   lyophilized powder
impurities   ≤0.0005% Phosphorus (P)
  ≤0.1% Insoluble matter
ign. residue   <0.1%
anion traces   chloride (Cl-): ≤0.05%
  sulfate (SO42-): ≤0.05%
cation traces   Al: ≤0.0005%
  Ca: ≤0.001%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Mg: ≤0.001%
  NH4+: ≤0.05%
  Na: ≤0.01%
  Pb: ≤0.001%
  Zn: ≤0.0005%

Description

Application

Used for cell separation and organelle isolation.

Ficoll® 400 is used together with sodium diatrizoate (Paque) to create Ficoll-Paque, a density mixture, used for the separation of cells and organelles, especially lymphocytes and monocytes from blood. Ficoll 400® may be used for the development of new cell and organelle separation systems. Ficoll 400 is used in tissue engineering and macromolecular conformation research for the development, evaluation and use of macromolecular crowding (MMC) systems and configurations.

Other Notes

A nonionic synthetic polymer of sucrose.

Packaging

25, 100 g in poly bottle

Legal Information

Ficoll is a registered trademark of GE Healthcare

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Protocols

FICOLL 400*

Ficoll 400 is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400 is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400 is v...
Keywords: Cell culture, Centrifugation, Culture media, Dialysis, Electrophoresis, Fractionation, Growth factors, Nucleic acid hybridization, Separation, Southern blot, Substitutions

Peer-Reviewed Papers
15

References

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