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F1638 Sigma

Anti-Pig IgG (whole molecule)−FITC antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Porcine IgG (whole molecule)−FITC antibody produced in rabbit, Anti-Swine IgG (whole molecule)−FITC antibody produced in rabbit

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Properties

Related Categories Alphabetical Index, Antibodies, FITC Antibodies, PH-PI, Pig/Swine Secondary Antibodies and Conjugates,
biological source   rabbit
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
storage condition   protect from light
application(s)   direct immunofluorescence: 1:32
conjugate   FITC conjugate
storage temp.   −20°C

Description

Features and Benefits

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Immunogen

Purified pig IgG

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-Pig IgG (whole molecule)-FITC antibody is suitable for use in direct immunofluorescence (1:32).

Rabbit polyclonal anti-Pig IgG (whole molecule)−FITC antibody may be used to detect and quantitate the level of IgG in pig serum and biological fluids by fluorescent techniques. It may also be used as a secondary antibody in assays that use pig IgG as the primary antibody.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates various cellular immunological responses. Pig IgGs are involved in mediating several pathogenic responses, including the response to Trichinella infections . Anti-Pig IgG (whole molecule)−FITC antibodies can be used to study the detection and localization of pig IgGs. The product is specific for pig IgGs.

Pig IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in pig serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.

Rabbit polyclonal anti-Pig IgG (whole molecule)−FITC antibody reacts with pig IgG in vitro, in pig serum and biological fluids.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Related Content

Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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