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  • F1777 - Monoclonal Anti-HLA-DQ−FITC antibody produced in mouse

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F1777 Sigma

Monoclonal Anti-HLA-DQ−FITC antibody produced in mouse

clone HK19, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-HLA-DQ

Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood,
species reactivity   human
application(s)   flow cytometry: 10 μL using 1 × 106 cells
clone   HK19, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2b
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... HLA-DQB1(3119)
biological source   mouse
conjugate   FITC conjugate

Description

Immunogen

GM-1500 human tumor cell line.

Antigen Background

HLA-DQ molecules are bimolecular glycoprotein complexes of 29 kDa and 33 kDa components. The HLA-DQ antigens are present on peripheral blood lymphocytes and tonsillar and splenic mononuclear cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Specificity

Recognizes MHC Class II molecules that are preferentially expressed on the surface of B lymphocytes. The antibody reacts weakly with resting or ConA-activated T cells, monocytes, granulocytes, and erythrocytes.

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

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