F1804 Sigma

Monoclonal ANTI-FLAG® M2 antibody produced in mouse

1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution

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Synonym: Anti-ddddk, Anti-dykddddk, flag antibody

Properties

Related Categories Alphabetical Index, Antibodies, Clone Index, FK-FO, FLAG Antibodies,
antibody form   affinity isolated antibody
clone   M2, monoclonal
form   buffered aqueous solution
concentration   1 mg/mL
isotype   IgG1
immunogen sequence   DYKDDDDK
shipped in   wet ice
storage temp.   −20°C

Description

Frequently Asked Questions

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Application

For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation, immunohistochemisty, and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.

Browse additional application references in our FLAG® Literature portal.

Features and Benefits

Antibody Bioguarantee
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General description

The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.

Immunogen

FLAG; peptide sequence DYKDDDDK

Preparation Note

Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk

Specificity

Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

Physical form

Contains 50% glycerol in phosphate buffered saline.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

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