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F1902 Sigma

Monoclonal Anti-HLA-DR−FITC antibody produced in mouse

clone HK14, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-HLA-DR

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood,
species reactivity   human
application(s)   flow cytometry: 10 μL using 1 × 106 cells
clone   HK14, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... HLA-DRA(3122), HLA-DRB1(3123)
biological source   mouse
conjugate   FITC conjugate

Description

Immunogen

GM-1500 human tumor cell line

Antigen Background

HLA-DR is located on chromosome 6. It is composed of a heavy and light chain, which are two non-covalently associated subunits of molecular weight 33-36 kDa and 24-30 kDa, respectively. These class II antigens are expressed primarily on B cells, and on monocytes and activated T cells, and function in cell-cell recognition in regulation of the immune response.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Specificity

Recognizes HLA-DR, a product of the human major histocompatibility complex. The antibody is specific for a non-polymorphic HLA-DR determinant.

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

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