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F1926 Sigma

Monoclonal Anti-Fas Ligand antibody produced in mouse

~1 mg/mL, clone Alf1.2, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-CD95L, Anti-FasL

Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology,
species reactivity   human
application(s)   flow cytometry: 1-2 μg/test using cultured KFL9 cells (human chronic myelogenous leukemia cell line K562, stably-transfected with human Fas Ligand)
  immunoprecipitation: suitable
  indirect ELISA: suitable
  microarray: suitable
clone   Alf1.2, monoclonal
concentration   ~1 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 24-40 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FASLG(356)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant soluble active extracellular domain of human Fas ligand.

General description

The Fas ligand (Fas ligand, FasL, CD95L) is an integral membrane protein and is a type II transmembrane glycoprotein. FasL is a member of the TNF family, which includes TNFα, α and β chains of lymphotoxin (LT), CD40 ligand and CD30 ligand. It plays an essential role in the activation-induced cell death of T lymphocytes, the maintenance of immunologically privileged anatomical sites, and cytotoxic mechanisms for a variety of different cells.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Application

Anti-Fas Ligand antibody is suitable for flow cytometry in 1-2μg/test concentraion using cultured KFL9 cells (human chronic myelogenous leukemia cell line K562, stably-transfected with human Fas Ligand), immunoprecipitation, indirect ELISA, and microarray.

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Biochem/physiol Actions

FasL has four potential N-glycosylation sites which appear to be variably used. It is expressed on activated T cells, whereas Fas is expressed on various types of cells. Engagement of Fas by its ligand, results in the rapid induction of programmed cell death (PCD) in susceptible cells. This process bypasses the usual long sequence of signaling enzymes and immediately activates preexisting caspases. It is also expressed on a number of lymphoma cell lines, on Epstein-Barr virus-transformed B lymphoblasts, and on a proportion of activated B and T cells.

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