Synonym: CIG, Cold insoluble globulin
|Related Categories||2D Cell Culture, Adhesion, Attachment, and Matrix Factors, Adhesion, Attachment, and Matrix Factors for Stem Cell Expansion, Application Index, Attachment Factors,|
|surface coverage||1‑5 μg/cm2|
|solubility||H2O: soluble1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)|
|suitability||suitable for cell culture|
|shipped in||dry ice|
|Gene Information||human ... FN1(2335)|
Live Chat and Frequently Asked Questions are available for this Product.
Protein homogeneity is evaluated by immunoelectrophoresis.
Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells
Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/ml. Optimal concentration depends on cell type as well as the application or research objectives.
Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.
Source material tested negative for HBsAg and HIV antibody.
Store reconstituted solution in working aliquots at -20°C or lower.
Lyophilized from 0.05 M Tris buffered saline, pH 7.5
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Certificate of Analysis
Certificate of Origin
Freezing and thawing of reconstituted fibronectin is not recommended as breakdown of protein will occur.
BioFiles 2008, 3.8, 9.
Keywords: Phase transitions
Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein composed of two nearly identical disulfidebound polypeptides of molecular weight 220 kDa. Cellular fibronectin is structurally...
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Despite their complexity, blood and plasma are abundant biological resources for the discovery of drug targets and biomarkers for human disease. It is estimated that plasma may contain as many as 40,...
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Traditionally, cancer has been viewed as a disease in which environmental or endogenous events induce mutations to critical oncogenes and tumor suppressor genes within a normal cell. The clinical man...
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Keywords: Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Catalysis, Cell culture, Cell division, Cell proliferation, Clinical, DNA replication, Degradations, Eliminations, Environmental, Gastrointestinal, Gene expression, Growth factors, Ligands, Metabolic Pathways, Phosphorylations, Poisons, Transcription, Transfection, transformation
TGF-β mediates PTEN suppression and cell motility through calcium-dependent PKC-α activation in pancreatic cancer cells. Jimmy Y. C. Chow et al Am. J. Physiol. Gastrointest. Liver Physiol. 294, G899-905, (2009)
Integrin alpha1beta1 regulates matrix metalloproteinases via P38 mitogen-activated protein kinase in mesangial cells: implications for Alport syndrome. Cosgrove, D Am. J. Pathol. 172(3), 761-73, (2008)
Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method. Michelle A. Digman et al Biophys. J. 96, 707-16, (2009)
The phosphorylation of vascular endothelial growth factor receptor-2 (VEGFR-2) by engineered surfaces with electrostatically or covalently immobilized VEGF. Anderson SM Biomaterials 30(27), 4618-28, (2009)
Mesenchymal stem cells exhibit firm adhesion, crawling, spreading and transmigration across aortic endothelial cells: effects of chemokines and shear. Chamberlain G, Smith H, Rainger GE, et al. PLoS ONE 6(9), e25663, (2011)
Fibronectin and fibronectin fragments. Akiyama, S. and Yamada, K., Haralson, M. and Hassell, J.R., ed. Extracellular Matrix: A Practical Approach New York , (1995), 183
Growth factors and other nonnutrient factors. Butler, M., Butler, M and Dawson, M.,ed. Cell Culture Labfax Oxford, England , (1992), 160
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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