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F2006 Sigma

Fibronectin from human plasma

lyophilized powder, BioReagent, suitable for cell culture

Synonym: CIG, Cold insoluble globulin

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Description

Frequently Asked Questions

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Components

Fibronectin is an adhesive glycoproteins found in both the cell surface and in plasma.

Application

Fibronectin from human plasma can be used with epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Caution

The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.

Preparation Note

This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C. In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.

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Safety & Documentation

Safety Information

WGK Germany 
3

Protocols & Articles

Articles

Fibronectin Cell Attachment Protocol

Freezing and thawing of reconstituted fibronectin is not recommended as breakdown of protein will occur.
George Sitterley
BioFiles 2008, 3.8, 9.
Keywords: Phase transitions, Tools

Fibronectin

Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein composed of two nearly identical disulfidebound polypeptides of molecular weight 220 kDa. Cellular fibronectin is structurally...
George Sitterley
BioFiles 2008, 3.8, 8.
Keywords: Adhesion, Cell culture, transformation

Plasma Derived Proteins and Enzymes

Despite their complexity, blood and plasma are abundant biological resources for the discovery of drug targets and biomarkers for human disease. It is estimated that plasma may contain as many as 40,...
BioFiles 2006, 1.5, 2.
Keywords: Applications, Gas chromatography, Type

The Cancer Stem Cell Hypothesis

Traditionally, cancer has been viewed as a disease in which environmental or endogenous events induce mutations to critical oncogenes and tumor suppressor genes within a normal cell. The clinical man...
Vicki Caligur
BioFiles 2008, 3.5, 4.
Keywords: Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Catalysis, Cell culture, Cell division, Cell proliferation, Clinical, DNA replication, Degradations, Eliminations, Environmental, Events, Gastrointestinal, Gene expression, Growth factors, Ligands, Metabolic Pathways, Phosphorylations, Poisons, Transcription, Transfection, transformation

Peer-Reviewed Papers

References

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Culture and analysis of circulating fibrocytes. Quan TE and Bucala R Methods Mol. Med. 135, 423-34, (2007)

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The performance of laminin-containing cryogel scaffolds in neural tissue regeneration. Jurga, M., et al. Biomaterials 32, 3423-34, (2011)

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Molecular interplay between endostatin, integrins, and heparan sulfate. Faye, C., et al. J. Bacteriol. 284, 22029-40, (2009)

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Ail Binding To Fibronectin Facilitates Yersinia Pestis Binding To Host Cells And Yop Delivery Tsang, T.M., et al. Infect. Immun. 78, 3358-68, (2010)

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Fibronectin binds and enhances the activity of bone morphogenetic protein 1. Huang G, Zhang Y, Kim B, Ge G, et. al. J. Biol. Chem. 284, 15879-25888, (2009)

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Extracellular stimulation of mammalian neurons through repetitive activation of Na+ channels by weak capacitive currents on a silicon chip. Schoen, I., and Fromherz, P. J. Autoimmun. 100, 346-57, (2008)

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Characterizing endothelial cells derived from the murine embryonic stem cell line CCE. Fathi F, Kermani AJ, Pirmoradi L, et al. Rejuvenation Res. 11(2), 371-8, (2008)

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A polydimethylsiloxane-polycarbonate hybrid microfluidic device capable of generating perpendicular chemical and oxygen gradients for cell culture studies. Chang CW, Cheng YJ, Tu M, et al. Lab Chip 14(19), 3762-72, (2014)

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Isolation of a bovine plasma fibronectin-containing complex which inhibits the expression of bovine leukemia virus p24. van den Heuvel MJ, Jefferson BJ, and Jacobs RM J. Virol. 79(13), 8164-70, (2005)

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Stoichiometry of molecular complexes at adhesions in living cells. Digman MA, Wiseman PW, Choi C, et al. Proc. Natl. Acad. Sci. U. S. A. 106(7), 2170-5, (2009)

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Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method. Michelle A. Digman et al Biophys. J. 96, 707-16, (2009)

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The phosphorylation of vascular endothelial growth factor receptor-2 (VEGFR-2) by engineered surfaces with electrostatically or covalently immobilized VEGF. Anderson SM Biomaterials 30(27), 4618-28, (2009)

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Local gene delivery from ECM-coated poly(lactide-co-glycolide) multiple channel bridges after spinal cord injury. De Laporte L Biomaterials 30(12), 2361-8, (2009)

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High-resolution quantification of focal adhesion spatiotemporal dynamics in living cells. Berginski ME, Vitriol EA, Hahn KM, et al. PLoS ONE 6(7), e22025, (2011)

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TGF-β mediates PTEN suppression and cell motility through calcium-dependent PKC-α activation in pancreatic cancer cells. Jimmy Y. C. Chow et al Am. J. Physiol. Gastrointest. Liver Physiol. 294, G899-905, (2009)

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Mesenchymal stem cells exhibit firm adhesion, crawling, spreading and transmigration across aortic endothelial cells: effects of chemokines and shear. Chamberlain G, Smith H, Rainger GE, et al. PLoS ONE 6(9), e25663, (2011)

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Regulation of EphB2 activation and cell repulsion by feedback control of the MAPK pathway. Alexei Poliakov et al J. Cell Biol. 183, 933-47, (2008)

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Integrin alpha1beta1 regulates matrix metalloproteinases via P38 mitogen-activated protein kinase in mesangial cells: implications for Alport syndrome. Cosgrove, D Am. J. Pathol. 172(3), 761-73, (2008)

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Inductive specification and axonal orientation of spinal neurons mediated by divergent bone morphogenetic protein signaling pathways. Jeanette C Perron et al Neural Dev. 6, 36, (2011)

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Overexpression of cell surface cytokeratin 8 in multidrug-resistant MCF-7/MX cells enhances cell adhesion to the extracellular matrix. Fang Liu et al Neoplasia 10, 1275-84, (2008)

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Characterization of the interaction between Actinin-Associated LIM Protein (ALP) and the rod domain of alpha-actinin. Klaavuniemi T BMC Cell Biol. 10, 22, (2009)

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Astrocyte-specific genes are generally demethylated in neural precursor cells prior to astrocytic differentiation. Hatada I PLoS ONE 3(9), e3189, (2008)

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Smad4-dependent pathways control basement membrane deposition and endodermal cell migration at early stages of mouse development. Costello I, Biondi CA, Taylor JM, et al. BMC Dev. Biol. 9, 54, (2009)

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OspA-CD40 dyad: ligand-receptor interaction in the translocation of neuroinvasive Borrelia across the blood-brain barrier. Lucia Pulzova et al Sci. Rep. 1, 86, (2011)

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Direct interaction of tumor suppressor CEACAM1 with beta catenin: identification of key residues in the long cytoplasmic domain. Jin L Exp. Biol. Med. (Maywood.) 233(7), 849-59, (2008)

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Fibroblast growth factor receptor 1 is a key inhibitor of TGFβ signaling in the endothelium. Chen PY, Qin L, Tellides G, et al. Sci. Signal. 7(344), ra90, (2014)

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Fibronectin and fibronectin fragments. Akiyama, S. and Yamada, K., Haralson, M. and Hassell, J.R., ed. Extracellular Matrix: A Practical Approach New York , (1995), 183

Growth factors and other nonnutrient factors. Butler, M., Butler, M and Dawson, M.,ed. Cell Culture Labfax Oxford, England , (1992), 160

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