F2006 Sigma

Fibronectin from human plasma

lyophilized powder, BioReagent, suitable for cell culture

Synonym: CIG, Cold insoluble globulin

Description

Frequently Asked Questions

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Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Application

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells

Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/ml. Optimal concentration depends on cell type as well as the application or research objectives.

Caution

Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.

Quality

Source material tested negative for HIV, hepatitis B and hepatitis C

Reconstitution

Store reconstituted solution in working aliquots at -20°C or lower.

Physical form

Lyophilized from 0.05 M Tris buffered saline, pH 7.5

Price and Availability


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