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F2043 Sigma

Monoclonal Anti-β-Tubulin−FITC antibody produced in mouse

clone TUB 2.1, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-β-Tubulin

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins,
species reactivity   chicken, human, plant, frog, wheat, rabbit, bovine, hamster, moth, mouse, sea urchin, rat
application(s)   direct immunofluorescence: 1:25 using cultured BHK cells
clone   TUB 2.1, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 55 kDa
shipped in   dry ice
storage condition   protect from light
storage temp.   −20°C
biological source   mouse
conjugate   FITC conjugate

Description

Immunogen

tubulin from rat brain.

General description

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Tubulin is a heterodimer, which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. The most studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections.

Application

FITC-β tubulin antibody may be used for detection ajnd localization of β-tubulin by direct labeling in a variety of eukaryotic cells ranging from human embryonic kidney cells (HEK293T) to cells of azuki bean epicotyl. by immunohistochemical and immunocytochemical assays.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of dist...
Keywords: Enzyme-linked immunosorbent assay, Gene expression, Western blot

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Peer-Reviewed Papers
15

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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