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F2047 Sigma

Monoclonal Anti-c-Myc−FITC antibody produced in mouse

clone 9E10, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-c-Myc

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Nuclear Proteins,
species reactivity   human
application(s)   immunocytochemistry: 5.0 μg/mL using mammalian cells transfected with c-Myc tagged fusion protein, fixed with paraformaldehyde.
clone   9E10, monoclonal
concentration   5 μg/mL (suitable for immunoflourescence)
antibody form   purified immunoglobulin
form   buffered aqueous solution
shipped in   dry ice
storage condition   protect from light
storage temp.   −20°C
Gene Information   human ... MYC(4609)
biological source   mouse
conjugate   FITC conjugate

Description

Immunogen

synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein.

General description

The mouse monoclonal Anti-c-Myc FITC conjugate is an antiblody isolated from ascites fluid and conjugated to the flourescein isothiocyanate iosmer I (FITC). This antibody recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed n or C-terminal to the fusion protein.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Specificity: N-terminal or C-terminal c-Myc (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) tagged fusion proteins.

Application

Suitable for detection of c-Myc tagged fusion proteins in transfected mammalian cells by Immunofluorescence and immunocytochemistry.

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