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  • F2266 - Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–FITC antibody produced in sheep

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F2266 Sigma

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–FITC antibody produced in sheep

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, FITC Antibodies, MO-MR,
biological source   sheep
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
application(s)   direct immunofluorescence: 1:128
conjugate   FITC conjugate
storage temp.   2-8°C

Description

Features and Benefits

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Other Notes

Antibody adsorbed with rat serum proteins.

Immunogen

Mouse IgG

Preparation Note

Adsorbed to reduce background staining with rat samples.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

Anti-Mouse IgG (whole molecule) F(ab’)2 fragment-FITC antibody is suitable for use in flow cytometry, immunohistochemistry, indirect immunofluorescence, and immunohistochemistry .

General description

IgGs are glycoprotein antibodies that regulate immunological defense mechanisms such as phagocytosis and inflammatory responses . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (whole molecule) F(ab’)2 fragment-FITC antibodies are specific for mouse IgG subclasses G1, G2a, G2b, and G3 and do not react with serum proteins in rat.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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