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F2442 Sigma

Fetal Bovine Serum

USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

Synonym: FBS

Purchase

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Analysis Note

Endotoxin and hemoglobin tested

Application

FBS derived from clotted blood is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.

General description

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Linkage

Tired of managing your serum needs? We can assist with your serum planning. Please visit the Serum Planner for more information.

Protocols & Applications

Aseptic Techniques Video

Cryostor Cryopreservation Video Protocol

Mycoplasma PCR Detection Kit Video Protocol

Price and Availability


All labs need water

Stericup and Steritop Filter Units
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Detection of Endogenous Pathway Activity in Novel Reporter Cell Lines

CompoZr® ZFN (zinc finger nuclease) technology was used to insert the sequence for fluorescent proteins adjacent to endogenous genes for signal transduction proteins to create novel reporter cell lin...
Andrey Samsonov, Senior Scientist; Hongyi Zhang, Senior Scientist; Fan Zhang, Senior Scientist; Nathan Zenser, Senior Scientist; John Fetter, Associate Research Fellow; Dmitry Malkov, Principal Investigator
Biowire Spring 2012, 13–18
Keywords: Angiogenesis, Antitumor agents, Apoptosis, Cell signaling, Gene expression, Growth factors, Immunostaining, Ligands, Recombination, Transcription, Transduction, Type

The MISSION® Target ID Library for Human miRNA Target Identification and Discovery

One of the main problems miRNA researchers face today is global identification of gene targets that are functionally regulated by their miRNA of interest. Unfortunately, bioinformatic predictions are...
Keywords: AGE, Amplification, Apoptosis, Cancer, Cell culture, Cloning, Degradations, Detection methods, Digestions, Electrophoresis, Environmental, Fractionation, Gel electrophoresis, Gene expression, Genomics, Immunoprecipitation, Melting, Methods, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA immunoprecipitation, Reductions, Sequences, Sequencing, Support, Transfection, Western blot, transformation

Protocols

Cell Quantification

For the majority of manipulations using cell cultures, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to...
Cook Book Sept 2010 Volume 12
Keywords: Carcinogens, Cryopreservation

Cryopreservation of Cell Lines

The protocol below describes the use of passive methods involving an electric -80°C freezer for the cryopreservation of cell cultures. ECACC routinely use a programmable rate controlled freezer. This...
Cook Book Volume 12
Keywords: Cell culture, Cryopreservation, Phase transitions

Lentiviral Transduction Protocol

Prepare mammalian cells so that they are growing exponentially and are no more than 70-80% confluent before transduction.
Keywords: Cell culture, Gene expression, Molecular biology, Transduction

Resuscitation of Frozen Cell Lines

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
Fundamental Techniques in Cell Culture Laboratory Handbook - 2nd Edition
Keywords: Centrifugation, PAGE

Subculture of Adherent Cell Lines

Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be sub cultured in order to preve...
Cook Book Sept 2010 Volume 12, Fundamental Techniques in Cell Culture Laboratory Handbook-2nd Edition
Keywords: Adhesion

Subculture of Semi-Adherent Cell Lines

Some cultures grow as a mixed population (e.g. B95-8 - marmoset) where a proportion of cells do not attach to the tissue culture fl ask and remain in suspension. Therefore to maintain this heterogene...
Cook Book Sept 2010 Volume 12
Keywords: Cell culture

Subculture of Suspension Cell Lines

In general terms cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastoid cell lines). For these types of ce...
Cook Book Sept 2010 Volume 12
Keywords: Centrifugation, Growth factors

Testing for Mycoplasma by Indirect DNA Stain

DNA staining methods such as indirect Hoechst staining techniques are quick with results available within 72 hours which compares favourably with 4 weeks for detection by culture. Staining of culture...
Cook Book Volume 12
Keywords: Cell culture, Detection methods, Evaporation, Indicators, Polymerase chain reaction

Peer-Reviewed Papers
15

References

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