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F2518 Sigma

Fibronectin from human foreskin fibroblasts

lyophilized powder, BioReagent, suitable for cell culture

Synonym: CIG, Cold insoluble globulin

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Description

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Application

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells

Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/ml. Optimal concentration depends on cell type as well as the application or research objectives.

Biochem/physiol Actions

Adhesion glycoprotein of the extracellular matrix isolated from human foreskin fibroblasts maintained in serum-free medium. Exists as a dimer with the A chain and the B chain containing the type III connecting segment (IIIcs) region. Different forms of fibronectin appear to be generated from tissue-specific splicing of fibronectin mRNA, transcribed from a single gene. Multiple domains of fibronectin show binding affinities for collagen, fibrin, heparin and specific cell membrane receptors. The most notable domain, Arg-Gly-Asp (RGD), is recognized by integrins and mediates cell adhesion. Involved in widespread interactions and functions such as the attachment and migration of many cell types, cytoskeletal assembly, tyrosine phosphorylation, and metastasis.
Plasma fibronectin molecular weight (heterodimeric): 450 kDa; Cellular fibronectin: 550 kDa
Differs from plasma fibronectin in the presence of additional polypeptide segments and in altering morphology of transformed cells and hemagglutination.

Caution

Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.

Packaging

0.5 mg in serum bottle

Reconstitution

Store reconstituted solution in working aliquots at -20°C or lower.

Physical form

Lyophilized in CAPS buffered saline

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Fibronectin Cell Attachment Protocol

Freezing and thawing of reconstituted fibronectin is not recommended as breakdown of protein will occur.
George Sitterley
BioFiles 2008, 3.8, 9.
Keywords: Phase transitions

Fibronectin

Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein composed of two nearly identical disulfidebound polypeptides of molecular weight 220 kDa. Cellular fibronectin is structurally...
George Sitterley
BioFiles 2008, 3.8, 8.
Keywords: Adhesion, Cell culture, transformation

The Cancer Stem Cell Hypothesis

Traditionally, cancer has been viewed as a disease in which environmental or endogenous events induce mutations to critical oncogenes and tumor suppressor genes within a normal cell. The clinical man...
Keywords: Apoptosis, Asymmetric synthesis, Cancer, Catalysis, Cell culture, Cell division, Cell proliferation, Cell signaling, Clinical, Degradations, Environmental, Gastrointestinal, Gene expression, Ligands, Phosphorylations, Poisons, Transcription, Transfection, transformation

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Keywords: Angiogenesis, Cancer, Eliminations, Gene expression, Growth factors, Ligands, Metabolic Pathways, Phosphorylations

Peer-Reviewed Papers
15

References

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