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F2637 Sigma

Ficoll® 400

BioXtra, for molecular biology, lyophilized powder

Synonym: Polysucrose 400

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Properties

Related Categories Biochemicals, Biochemicals and Reagents, Carbohydrates for Protein and Enzyme Stabilization, Cell Biology, Core Bioreagents,
grade   for molecular biology
product line   BioXtra
form   lyophilized powder
foreign activity   DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected
storage temp.   room temp

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Used for cell separation and organelle isolation.

Suitable for
• hybridization experiments to reduce non-specific binding of labeled probes to the membrane
• preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
• separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
• producing density gradients to separate subcellular components
• vitrification of cell and tissue samples.
• to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).

Other Notes

A nonionic synthetic polymer of sucrose.

Features and Benefits

• free from DNases, RNases, phosphatases and proteases
• preserves functional and morphological integrity of the separated cells

General description

Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.

Legal Information

Ficoll is a registered trademark of GE Healthcare

Price and Availability


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Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Protocols

FICOLL 400*

Ficoll 400 is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400 is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400 is v...
Keywords: Cell culture, Centrifugation, Culture media, Dialysis, Electrophoresis, Fractionation, Growth factors, Nucleic acid hybridization, Separation, Southern blot, Substitutions

Peer-Reviewed Papers
15

References

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