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F2637 Sigma

Ficoll® 400

BioXtra, for molecular biology, lyophilized powder

Synonym: Polysucrose 400

Purchase

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Used for cell separation and organelle isolation.

Suitable for
• hybridization experiments to reduce non-specific binding of labeled probes to the membrane
• preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
• separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
• producing density gradients to separate subcellular components
• vitrification of cell and tissue samples.
• to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).

Other Notes

A nonionic synthetic polymer of sucrose.

Features and Benefits

• free from DNases, RNases, phosphatases and proteases
• preserves functional and morphological integrity of the separated cells

General description

Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.

Legal Information

Ficoll is a registered trademark of GE Healthcare

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
Is Product F2637, Ficoll, the same as Ficoll 400?
Yes. It is Ficoll 400.  This product is graded molecular biology grade and is free of any nucleases
Can Product F2637, Ficoll, be used as a component of a DNA loading buffer?
This product can be used in a DNA loading buffer with the following recipe to prepare a 6X stock: 0.25% bromophenol blue 0.25% xylene cyanol FF 15% Ficoll
What is the difference between Ficoll 400 and Ficoll PM 400?
The difference between Ficoll 400 and Ficoll PM 400 is the PM 400 has stricter specifications on the number of microorganisms/g and the endotoxin level is < 0.125 EU/ml
Do you have a protocols for use of Product F2637, Ficoll, in various applications?
Please check the product information sheet for F4375 which has a detailed protocol. Here is the link to that document: http://www.sigmaaldrich.com/sigma-aldrich/product_information_sheet/f4375pis.pdf
Can you use Product F2637, Ficoll, to isolate mononuclear cells?
When trying to isolate mononuclear cells you can use this product but we offer Histopaque that is a mixture of Ficoll and Hypaque. For humans, we offer H8889, Histopaque®-1077 Hybri-Max™ or 1077-1, Histopaque®-1077. For rodents we offer 1083-1, Histopaque®-1083. For isolation of neutrophils, we offer 1119-1, Histopaque®-1119.  Here are the links to the information sheets for those four products:       http://www.sigmaaldrich.com/sigma/product%20information%20sheet/h8889pis.pdf http://www.sigmaaldrich.com/sigma/general%20information/1077.pdf http://www.sigmaaldrich.com/sigma/general%20information/1083-1pis.pdf http://www.sigmaaldrich.com/sigma/general%20information/1119.pdf
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Protocols

FICOLL 400*

Ficoll 400 is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400 is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400 is v...
Keywords: Cell culture, Centrifugation, Culture media, Dialysis, Electrophoresis, Fractionation, Growth factors, Nucleic acid hybridization, Separation, Southern blot, Substitutions

Peer-Reviewed Papers
15

References

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