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F2637 Sigma

Ficoll® 400

BioXtra, for molecular biology, lyophilized powder

Synonym: Polysucrose 400

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Properties

Related Categories Biochemicals, Cell Biology, Core Bioreagents, Density Gradient Media, General Reagents,
grade   for molecular biology
product line   BioXtra
form   lyophilized powder
foreign activity   DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected
storage temp.   room temp

Description

Frequently Asked Questions

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Application

Used for cell separation and organelle isolation.

Suitable for
• hybridization experiments to reduce non-specific binding of labeled probes to the membrane1
• preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
• separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
• producing density gradients to separate subcellular components
• vitrification of cell and tissue samples.2
• to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).3

Other Notes

A nonionic synthetic polymer of sucrose.

Features and Benefits

• free from DNases, RNases, phosphatases and proteases
• preserves functional and morphological integrity of the separated cells

General description

Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.

Legal Information

Ficoll is a registered trademark of GE Healthcare

Price and Availability


Genomics Promo

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Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are tran...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Size-exclusion chromatography, Sterilizations

Protocols

FICOLL 400*

Ficoll 400 is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400 is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400 is v...
Keywords: Cell culture, Centrifugation, Culture media, Dialysis, Electrophoresis, Fractionation, Growth factors, Nucleic acid hybridization, Separation, Southern blot, Substitutions

Peer-Reviewed Papers

References

Set your institution to view full text papers.

1. Gradients of Eph-A6 expression in primate retina suggest roles in both vascular and axon guidance. Kozulin P, Natoli R, Madigan MC, et al. Mol. Vis. 15, 2649-62, (2009)

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2. Cryopreservation of cartilage cell and tissue for biobanking. Cetinkaya G and Arat S Cryobiology 63(3), 292-7, (2011)

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3. Optimization of isolated perfused/ventilated mouse lung to study hypoxic pulmonary vasoconstriction. Yoo HY, Zeifman A, Ko EA, et al. Pulm. Circ. 3(2), 396-405, (2013)

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Fractionation of rat ventricular nuclei. Jackowski, G, and Liew, C.C. Biochem. J. 188, 363-373, (1980)

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Purification of gibberellic acid-induced lysosomes from wheat aleurone cells. Gibson, R.A., and Paleg, L.G. J. Cell Sci. 22, 413, (1976)

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Preparation of extracts from yeast. Jazwinski, S.M Meth. Enzymol. 182, 154, (1990)

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A role for phosphatidic acid in the formation of "supersized" lipid droplets. Fei W PLoS Genet. 7(7), e1002201, (2011)

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MRI assessment of ischemic liver after intraportal islet transplantation. Naoaki Sakata et al Transplantation 87, 825-30, (2009)

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Detection of dynamic spatiotemporal response to periodic chemical stimulation in a Xenopus embryonic tissue. Kim Y, Joshi SD, Messner WC, et al. PLoS ONE 6(1), e14624, (2011)

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Activity of mevalonate pathway inhibitors against breast and ovarian cancers in the ATP-based tumour chemosensitivity assay. Knight, L.A., et al. BMC Biochem. 9, 38, (2009)

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