F2637 Sigma

Ficoll® 400

BioXtra, for molecular biology, lyophilized powder

Synonym: Polysucrose 400



Related Categories Biochemicals, Cell Biology, Core Bioreagents, Density Gradient Media, General Reagents,
grade   for molecular biology
product line   BioXtra
form   lyophilized powder
foreign activity   DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected
storage temp.   room temp


Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.


Used for cell separation and organelle isolation.

Suitable for
• hybridization experiments to reduce non-specific binding of labeled probes to the membrane
• preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
• separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
• producing density gradients to separate subcellular components

Other Notes

A nonionic synthetic polymer of sucrose.

Features and Benefits

• free from DNases, RNases, phosphatases and proteases
• preserves functional and morphological integrity of the separated cells

General description

Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.

Legal Information

Ficoll is a registered trademark of GE Healthcare

Price and Availability

Genomics Promo

Customers Also Viewed

Ficoll<SUP>®</SUP> 400

Type 400-DL, lyophilized powder

Ficoll<SUP>®</SUP> 400

lyophilized powder, Type 400-DL, BioReagent, suitable for cell culture

lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

Ficoll<SUP>®</SUP> 400

BioXtra, Type 400-DL, lyophilized powder

Ficoll<SUP>®</SUP> PM 400

Type 400

Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are tran...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Size-exclusion chromatography, Sterilizations



Ficoll 400 is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400 is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400 is v...
Keywords: Cell culture, Centrifugation, Culture media, Dialysis, Electrophoresis, Fractionation, Growth factors, Nucleic acid hybridization, Separation, Southern blot, Substitutions

Related Products

related product

Product #


Add to Cart

I9516 2-Propanol, BioReagent, for molecular biology, ≥99.5%
D8418 Dimethyl sulfoxide, BioReagent, for molecular biology, ≥99.9%
P4557 Phenol solution, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?