|Related Categories||Biochemicals, Cell Biology, Core Bioreagents, Density Gradient Media, General Reagents,|
|grade||for molecular biology|
|foreign activity||DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected|
|storage temp.||room temp|
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Used for cell separation and organelle isolation.
• hybridization experiments to reduce non-specific binding of labeled probes to the membrane
• preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
• separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
• producing density gradients to separate subcellular components
A nonionic synthetic polymer of sucrose.
• free from DNases, RNases, phosphatases and proteases
• preserves functional and morphological integrity of the separated cells
Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.
Ficoll is a registered trademark of GE Healthcare
The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are tran...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Size-exclusion chromatography, Sterilizations
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