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F2648 Sigma

Monoclonal Anti-CD11b−FITC antibody produced in mouse

clone ICRF44, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-CD11b

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
species reactivity   human
application(s)   flow cytometry: 10 μL using 1 × 106 cells
clone   ICRF44, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 165-170 kDa
shipped in   wet ice
storage condition   protect from light
storage temp.   2-8°C
Gene Information   human ... ITGAM(3684)
biological source   mouse
conjugate   FITC conjugate

Description

Immunogen

human monocytes.

General description

CD11b is an α/β heterodimeric glycoprotein. It belongs to the β2 integrin family and also referred as Mac-1, CR3, MO-1, and C3bi receptor.

Antigen Background

CD11b α-chain of the human CD11b/CD18 complex is an α/β heterodimeric glycoprotein which belongs to the β2 integrin family. It is also known as Mac-1, CR3, MO-1 and C3bi receptor. CD11b is expressed on the surface of circulating monocytes, granulocytes and certain NK cells and is also present in subsets of macrophages. It functions as a receptor for iC3b, clotting factor X, fibrinogen and ICAM-1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

Recognizes the CD11b α-chain of the human CD11b/CD18 complex. The antibody is useful for studies on cell adhesion and migration and for the detection of leukocyte adhesion deficiencies. It may be used to stain acetone-fixed cryostat sections or cell preparations. The epitope recognized by the antibody is formalin sensitive.
2nd Workshop: code no. M104
3rd Workshop: code no. 202 and 238
4th Workshop: code no. M47

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Application

Anti-CD11b-FITC antibody is suitable for flow cytometric analysis of CD11b expression in RA-induced differentiation of human promyelocytic leukemia cells. It is also suitable for flow cytometric analysis of spleen cells to study the beneficial effects of dietary consumption of n-3 polyunsaturated fatty acids (PUFA) and two selective estrogen receptor modulator (SERM) derivatives.

Features and Benefits

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Biochem/physiol Actions

Anti-CD11b-FITC antibody is expressed on the surface of circulating monocytes, granulocytes, and certain NK cells. It is also present on subsets of macrophages and in subcellular granules of granulocytes. Upon activation, it translocates to the surface. It forms a CD11b/CD18 complex to participate in a variety of cell-cell and cell-substrate interactions such as attachment and phagocytosis of particles coated with C3bi by granulocytes and macrophages and phagocytosis of opsonized pathogens. It also plays a role in the initiation of a coagulation protease cascade and in cell migration mechanisms.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
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Peer-Reviewed Papers
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References

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