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F2653 Sigma

Anti-Mouse IgG (Fab specific) F(ab′)2 fragment–FITC antibody produced in goat

flow cytometry grade, affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, FITC Antibodies, MO-MR,
biological source   goat
antibody form   affinity isolated antibody
grade   flow cytometry grade
clone   polyclonal
form   buffered aqueous solution
application(s)   flow cytometry: 1:100
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160
conjugate   FITC conjugate
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Features and Benefits

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General description

IgG, a monomer, is the principal antibody circulating in blood which assist in phagocytic destruction of foreign microorganism and is the only class of Ig that can cross the placenta in humans. Cell-sorting done by differential binding of anti-mouse IgG (Fab Specific) - FITC antibody separates tumour cells from macrophages. This antibody can be used to incubate somatic cell nuclear transfer (SCNT) rat embryos for immunocytochemical observation. It also can be used as a secondary antibody to study the dynamics of activated B lymphocytes in small intestine, Peyer’s patch germinal center as well as non-germinal center, spleen and the mesenteric lymph node. Anti-mouse IgG antibody reacts specifically with all mouse immunoglobulins.

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Immunogen

Purified Fab fragments of mouse IgG.

Preparation Note

Adsorbed to reduce background staining with bovine, horse or human samples.

Specificity

Binds all mouse Igs.
Useful when trying to avoid background staining due to the presence of Fc receptors.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What applications have product F2653, Anti-Mouse IgG (Fab specific) F(ab′)2 fragment–FITC antibody produced in goat, been used in?
Product F2653, Anti-Mouse IgG (Fab specific) F(ab′)2 fragment–FITC antibody produced in goat,  has been used in Flow Cytometry in the following literature references. Bergman, I. et al., Clinical Cancer Research., 7, 2050 (July 2001). Farsinejad, A. et al. Iranian Journal of Blood and Medicine, 2, Number 3, (Spring 2010). This antibody has been used in Immunofluorescencs in the following literature reference. Sushan, A., Hum. Reprod., 19 (9) : 1957-1967 (2004).
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Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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