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F2762 Sigma

Anti-Filamin antibody produced in goat

whole antiserum

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin, Antibodies to Cell and Organelle Proteins,
species reactivity   chicken
application(s)   indirect immunofluorescence: 1:40 using cultured chicken fibroblasts
  western blot: suitable using total chicken gizzard extract
clone   polyclonal
antibody form   whole antiserum
mol wt   antigen mol wt ~250 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   chicken ... LOC395260(395260)
biological source   goat
conjugate   unconjugated

Description

Immunogen

filamin from chicken gizzard.

Specificity

The product shows a uniform labeling of filamin associated with microfilament bundles (stress fibers) and membrane ruffles of cultured cells.

Application

Anti-Filamin antibody is suitable as a primary antibody in western blot analysis.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Features and Benefits

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Biochem/physiol Actions

Filamin, also known as actin-binding protein, is a large flexible V-shaped actin cross-linking protein present in vertebrates of both muscle and non-muscle cells. It has actin-binding domains at the ends of each arm. Filamin is a dimer of two large (280-kd) subunits, forming a flexible V-shaped molecule that crosslinks actin filaments into orthogonal networks. Filamin cross-links actin and promotes it’s aggregation and gelation.

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Safety & Documentation

Safety Information

WGK Germany  3
Protocols & Articles

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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