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F2772 Sigma

Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody produced in goat

flow cytometry grade, affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, FITC Antibodies, MO-MR,
biological source   goat
antibody form   affinity isolated antibody
grade   flow cytometry grade
clone   polyclonal
form   buffered aqueous solution
storage condition   protect from light
application(s)   flow cytometry: 1:100
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160
conjugate   FITC conjugate
storage temp.   −20°C

Description

Features and Benefits

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Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Immunogen

Purified Fc fragments of mouse IgG

Preparation Note

Adsorbed to reduce background with bovine, horse or human samples.
Useful when trying to avoid background staining due to the presence of Fc receptors.

Specificity

Goat polyclonal anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody isolated from goat antiserum by affinity chromatography reacts specifically with mouse Fc fragment. No cross reaction is observed with mouse Fab fragment. The antibodies are adsorbed to assure minimal cross reaction with human, horse and bovine proteins on tissue or cell preparations.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

For flow cytometry, a dilution of 1:100 may be used for labelling of human peripheral blood lymphocytes. A dilution of 1:50 was used to label human polymorphonuclear cells (PMNs). The antibody may be used for immunohistochemistry at a working dilution of 1:160. It was also used in immunocytochemistry using human embryonic stem cells.

Goat polyclonal anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody may be used as a reagent in flow cytometry, immunohistology and immunocytology offering sensitive and specific activity to mouse IgG and no cross reactivity with human, horse or bovine immunoglobulins.
Flow Cytometry: A minimum dilution of 1:100 is determined by flow cytometric analysis of 100 μl of human peripheral blood lymphocytes by indirect labeling. Immunohistology: A minimum dilution of 1:160 is determined by indirect immunofluorescence on formalinfixed, and paraffin-embedded human tonsil using mouse monoclonal anti-human IgG as the primary antibody.

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-mouse IgG is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I. The F(ab’)2 antibodies isolated from goat antiserum by affinity chromatography, react specifically with mouse IgG Fc; does not bind other mouse Igs. No cross reaction is observed with mouse Fab fragment. The antibody shows minimal cross reaction with human, horse and bovine proteins on tissue or cell preparations.

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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