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F2918 Sigma

Anti-Focal Adhesion Kinase (pp125FAK) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-FAK, Anti-Focal Adhesion Kinase (pp125FAK)

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Adhesion Molecules and Related Proteins,
species reactivity   rat, mouse, bovine, human
application(s)   immunocytochemistry: suitable
  immunoprecipitation: suitable using cultured human endothelial cell extract
  indirect immunofluorescence: 1:4,000 using cultured human endothelial cells
  microarray: suitable
  western blot: 1:2,000 using cultured human endothelial cells extract
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 125 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... PTK2(5747)
mouse ... Ptk2(14083)
rat ... Ptk2(25614)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to C-terminal region amino acids 1039-1052 of human, mouse, or rat focal adhesion kinase (pp125FAK).

General description

Focal Adhesion Kinase (FAK) is a non-receptor protein tyrosine kinase that is expressed ubiquitously. FAK is the critical in forming cell-substratum adhesions or focal adhesion complexes. FAK reportedly has multiple phosphorylation sites. Autophosphorylation of FAK at Tyr397 creates a binding site on FAK for Src-family kinases and is critical for maximum adhesion and migration responses. The phosphorylation of Tyr407 and Tyr861 is induced during epithelial-mesenchymal transition and further augmented during cell migration. FAK activation couples signal transduction via PI3K, CAS and Src. FAK signaling is involved in multiple functions such as proliferation, migration, survival and angiogenesis. Disruption of FAK signalling results in failure of adhesion to the substratum and anoikis (apoptosis) of epithelial cells which are anchorage dependent. FAK has been observed to be upregulated in human tumors such as neuroblastoma and is related to tumor cell survival and prognosis
Anti-Focal Adhesion Kinase (pp125 FAK) antibody recognizes FAK (Focal Adhesion Kinase) in human endothelial cultured cell line (125 kDa). An additional, weaker, lower molecular weight band may be seen.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Application

Anti-Focal Adhesion Kinase (pp125fak) antibody may be used at a working dilution of 1:2000 for detection by immunoblotting in human endothelial cells. The antibody is suitable for immunofluorescence at a working dilution of 1:4000. The antibody is suitable for immunoprecipitation, immunocytochemistry and protein microarray. The antibody had been used for detection by immunoblotting and immunoprecipitation in human breast cancer cell line, MCF-7.

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