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  • F3022 - Anti-β-Actin–FITC antibody, Mouse monoclonal

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F3022 Sigma

Anti-β-Actin–FITC antibody, Mouse monoclonal

clone AC-15, purified from hybridoma cell culture

Synonym: Monoclonal Anti-β-Actin

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Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin Isoforms,
species reactivity   guinea pig, canine, Hirudo medicinalis, feline, pig, carp, mouse, chicken, rabbit, sheep, rat, human, bovine
does not react with   Dictyostelium discoideum
application(s)   direct immunofluorescence: 1:250 using cultured human and chicken fibroblasts
clone   AC-15, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 42 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
biological source   mouse
conjugate   FITC conjugate

Description

Immunogen

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

General description

In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)

Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.

Monoclonal mouse anti-actin was used as a loading control for western blot analysis of rat liver protein lysates.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

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Biochem/physiol Actions

Actins is a eukaryotic protein. It belongs to the major cytoskeletal protein family and majorly associated with cell motility. It is ubiquitously present in cytoplasm and involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. It is expressed in mammals and birds and at least six isoforms have been identified.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles

Articles

Antibodies to Actin Gene Family Isoforms

The Actin Gene Family Actin and myosin are the two major cytoskeletal proteins implicated in cell motility. As constituents of many cell types, actin and myosin are involved in a myriad of cellular p...
Keywords: Western blot

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Peer-Reviewed Papers
15

References

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