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F3165 Sigma

Monoclonal ANTI-FLAG® M2 antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-ddddk, Anti-dykddddk

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags, FK-FO,
biological source   mouse
antibody form   purified immunoglobulin
clone   M2, monoclonal
form   buffered aqueous solution
application(s)   western blot (chemiluminescent): 10 μg/mL
isotype   IgG1
immunogen sequence   DYKDDDDK
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Features and Benefits

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Immunogen

FLAG; peptide sequence DYKDDDDK

Packaging

5 mg in poly bottle

Preparation Note

Dilute the antibody solution from 0.5-10 ug/mL in Tris Buffered Saline, pH 8.0, with 3% nonfat milk

Application

Antibody is recommended for use in immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, ELISA, electron microscopy, flow cytometry and supershift assays.

Browse additional application references in our FLAG® Literature portal.

General description

Anti Flag M2 antibody is used for the detection of Flag fusion proteins.This monoclonal antibody is produced in mouse and recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus. The antibody is also able to recognize FLAG at an internal site. M2, unlike M1 antibody is not Calcium dependent.
F1804 is an affinity purified, FLAG M2 antibody, increasing sensitivity in most applications.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Articles

Inhibition of Autoregulated TFG-ß

We studied autocrine transforming growth factor (TGF)beta signaling in kidney epithelium. Cultured proximal tubule cells showed regulated signaling that was high during log-phase growth, low during c...
Keywords: Growth factors

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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