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F3174 Sigma

Fpg Protein from Escherichia coli

≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, >20,000 units/mg protein

Synonym: Fapy DNA glycosylase, Formamidopyrimidine DNA glycosylase, MutM

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology,
recombinant   expressed in E. coli
assay   ≥90% (SDS-PAGE)
form   buffered aqueous glycerol solution
mol wt   mol wt 30.2 kDa (269 amino acids, predicted from the nucleotide sequence)
shipped in   wet ice
storage temp.   −20°C
Gene Information   Escherichia coli CFT073 ... mutM(1038243)
Escherichia coli K12 ... mutM(946765)

Description

Packaging

10 μg in poly bottle

Unit Definition

One unit will cleave 50% of 0.5 pmol of double-stranded DNA oligomer substrate (8-oxoguanine−mutated) in 10 min at 25 °C.

Physical form

Solution in 50% glycerol containing 50 mM potassium HEPES, pH 7.5, 1 mM DTT, 1 mM EDTA, and 200 mM NaCl.

Application

Fpg Protein from Escherichia coli has been used for the assessment of DNA oxidative damage using comet assay.

Biochem/physiol Actions

Formamidopyrimidine-DNA glycosylase (Fpg) cleaves double-stranded DNA containing the damaged base 8-oxo-7,8-dihydroguanine. It functions as an N-glycosylase and apurinic/apyrimidinic lyase.

General description

Formamidopyrimidine-DNA glycosylase (Fpg) is a DNA repair enzyme found in Escherichia coli, which contains one zinc atom. Proximal to its C-terminal, it contains a zinc-finger motif of CC/CC type.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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