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F3506 Sigma

N-Formyl-Met-Leu-Phe

≥97% (HPLC)

Synonym: Chemotactic peptide, N-Formyl-L-methionyl-L-leucyl-L-phenylalanine, fMLF, fMLP (misnomer but widely used)

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Chemotactic Peptides, Immune Cell Signaling and Blood, Immune System Regulation,
InChI Key   PRQROPMIIGLWRP-BZSNNMDCSA-N
assay   ≥97% (HPLC)
storage temp.   −20°C
Gene Information   human ... FPR1(2357)

Description

Amino Acid Sequence

NFor-Met-Leu-Phe

Packaging

5, 10 mg in glass insert

50, 100, 500 mg in glass bottle

Application

N-Formyl-Met-Leu-Phe has been used for:
• creating gradients in Zigmond chamber chemotaxis assay performed on neutrophils
• use in neutrophil chemotaix assay and
• determination of the involvement of MAPK-activating protein kinase-2 (MAPKAPK-2) and/or p38, in the signaling pathway of human polymorphonuclear leukocytes (PMNs) stimulated by N-Formyl-Met-Leu-Phe.

Biochem/physiol Actions

N-Formyl-Met-Leu-Phe induced chemotaxis in phosphoinositide 3-kinase γ (PI3K)γ-/- neutrophils, and promotes adhesion, polymerization of F-actin, Fcγ receptor-mediated phagocytosis and intracellular Ca2+ release. It acts as an inflammatory agent and activates polymorphonuclear leukocytes (PMNs) without the formation of 5-hydroxyicosatetraenoic acid (5-HETE) and leukotriene B4 (LTB4). Its effects on neutrophils and the inhibition of adenylate cyclase are inhibited by pertussis toxin.

Potent chemotactic peptide. Induces a metabolic burst in macrophages accompanied by an increase in respiratory rate, secretion of lysosomal enzymes, and production of superoxide anion.

Potent inducer of leucocyte chemotaxis and macrophage activator. Receptors that bind formylpeptides are found on phagocytic neutrophils and have recently been identified on cells of the intestinal mucosa.

General description

N-Formyl-Met-Leu-Phe is a chemotactin peptide, which functions on polymorphonuclear leukocytes (PMN), neutrophils etc.

Price and Availability


Amplified Detection

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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Description

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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