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F3543 Sigma

Fluorescent Brightener 28

used as a stain and brightening agent

Synonym: Calcofluor White M2R, Tinopal UNPA-GX

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

1, 5, 25 g in glass bottle

Application

It was used in the identification and study of the structure and biosynthesis of chitin, the second most abundant polysaccharide found in nature, from the freshwater sponge Spongilla lacustris. Fluorescent Brightener 28, which shows enhanced fluorescence when binding to chitin, was used to elucidate particular location of chitin in the skeletal structures.

General description

Fluorescent Brightener (Calcofluor White M2R) is used for the staining of fungi and as a viability stain. Industrial uses include use as a fluorescent brightening agent for cellulose and polyamide fabrics, paper and in detergents and soaps. It was used in the identification and study of the structure and biosynthesis of chitin, the second most abundant polysaccharide found in nature, from the freshwater sponge Spongilla lacustris. Fluorescent Brightener 28, which shows enhanced fluorescence when binding to chitin, was used to elucidate particular location of chitin in the skeletal structures.

Fluorescent Brightener 28 has been used for the staining of fungal cell walls. It has also been used for the staining of Candida albicans biofilm.

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How is Product F3543, Fluorescent Brightner 28, dissolved for use?
The product can be diluted in DMSO at a concentration of 50 mg/ml.  The product can be dissolved in water at a concentration of 1 mg/ml, although the use of 50% sodium hydroxide might be required to fully solubilize the product.
What are the excitation and emission wavelengths for Product F3543, Fluorescent Brightner 28?
Excitation is at 365 nm and emission at is at 435 nm.
Is there a reference for use of Product F3543, Fluorescent Brightner 28?
3 different staining applications are found in the following reference:  Clark, G; Staining Procedures, 4th Edition, Williams & Wilkins, Baltimore 1981 pages 46-48. This product is used to monitor removal or regeneration of plant cell walls. A protocol for using this product with plant protoplasts is described. D.W. Galbraith, Physiologia Plantarum, 53, 111-116 (1981). 
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles
Peer-Reviewed Papers
15

References

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