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F3648 Sigma

Anti-Fibronectin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Adhesion/Attachment/Matrix Factors, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
species reactivity   human
application(s)   indirect ELISA: 1:10,000 using human fibronectin
  indirect immunofluorescence: 1:400 using human foreskin cultured fibroblasts
  microarray: suitable
  western blot: 1:1,000 using human plasma fibronectin
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FN1(2335)
biological source   rabbit
conjugate   unconjugated
Featured Industry   Research Pathology

Description

Immunogen

fibronectin isolated from human plasma

General description

Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein. Cellular fibronectin exists as dimeric or cross-linked multimeric forms, made up of fibroblasts, epithelial and other cell types. The dimer form composed of two nearly identical ~250 kDa subunits linked covalently to the C-termini by a pair of disulfide bonds. 15 FN is present as dimeric form in plasma and secreted by hepatocytes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% BSA and 15 mM sodium azide

Specificity

The product shows no cross-reaction with laminin, vitronectin, collagen type IV, or chondroitin sulfate types A, B and C using a dot blot immunoassay.

Application

Anti-Fibronectin antibody is suitable for
• immunohistochemistry (IHC) analysis to study the activity of pancreatic stellate cells in chronic pancreatitis
• antibody blocking assays of bacterial cells to study the activity of fibronectin in the Yersinia pestis binding to host cells and Yop delivery
• staining of endothelial cells as primary antibody
• cell adhesion assays to study the effect of anti-human fibronectin antibodies on adhesion to endothelial cells
• immunoprecipitation and western blot analysis of NF-κB dependent mammary tumorigenesis study
• immunofluorescence staining to study the correlation between zyxin tails and fibrillar adhesions
• immunoblotting to study the expression levels of the pancreatic stellate cells (PSC) markers glial fibrillary acidic protein (GFAP), α-smooth muscle actin (α-SMA), collagen type I, and fibronectin in whole cell lysates
• ELISA of fibronectin secretion
• microarray analysis.

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Biochem/physiol Actions

FN plays important roles in cell adhesion, migration, growth and differentiation. It has capacity to bind various functionally important domains such as heparin, collagen/gelatin, and fibrin. It also stimulates human non-small cell lung carcinoma.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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