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F3662 Sigma

Monoclonal Anti-Phosphoserine−FITC antibody produced in mouse

clone PSR-45, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-Phosphoserine, Phospho Ser, Phospho serine, Phospho−Ser, Phospho−serine

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins,
biological source   mouse
antibody form   purified immunoglobulin
clone   PSR-45, monoclonal
form   buffered aqueous solution
storage condition   protect from light
application(s)   dot immunobinding: 1:64
  particle immunofluorescence: 1:32
isotype   IgG1
conjugate   FITC conjugate
shipped in   dry ice
storage temp.   −20°C

Description

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Immunogen

phosphoserine conjugated to Keyhole Limpet Hemocyanin (KLH).

Specificity

By ELISA and dot blot, the antibody reacts specifically with phosphorylated serine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphoserine-containing proteins. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody due to steric hindrance of the recognition site.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.01% thimerosal.

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