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F3777 Sigma

Monoclonal Anti-Actin, α-Smooth Muscle - FITC antibody produced in mouse

clone 1A4, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-Actin, α-Smooth Muscle, SMA

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Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology,
species reactivity   rabbit, mouse, guinea pig, chicken, goat, sheep, snake, human, frog, rat, canine, bovine
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human tonsil or appendix
clone   1A4, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
mol wt   antigen mol wt ~42 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)
biological source   mouse
conjugate   FITC conjugate

Description

Immunogen

N-terminal synthetic decapeptide of α-smooth muscle actin.

General description

The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide, as a preservative.

Application

Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.

Monoclonal Anti-Actin, α-Smooth Muscle - FITC antibody is suitable for:
• immunohistochemical (IHC) staining of heart, lung, kidney, and liver of mice to examine the PHD2 deficiency in internal organs
• immunostaining to analyze vascular smooth muscle cells/pericytes development in platelet-derived growth factor (PDGFR) and PDGFR- β-/- embryos
• immunofluorescent analysis of implanted cells, frozen tissue samples to study the similar levels of expression of SMaA and SM-MHC as established primary SMC lines
• western blots of lens epithelial cells to determine the distribution of α-SMA expression in lens epithelial cell

Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Features and Benefits

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Biochem/physiol Actions

Actin, a conserved eukaryotic protein, is the major cytoskeletal component which actively participates in cell motility. In mammals and birds, actin is expressed as six different isoforms. α-Smooth muscle actin, a smooth muscle cell-specific protein, is also known as aortic smooth muscle or α smooth muscle actin (α-SMA, SMactin, α-SM-actin, ASMA). It belongs to actin family, which are highly conserved proteins that play a role in cell motility, structure and integrity. It is predominantly found in cells of the smooth muscle lineages. It has been reported that α-smooth muscle actin was also found in certain non-muscle cells, in particular fibroblasts i.e. myofibroblasts.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles

Articles

Antibodies to Actin Gene Family Isoforms

The Actin Gene Family Actin and myosin are the two major cytoskeletal proteins implicated in cell motility. As constituents of many cell types, actin and myosin are involved in a myriad of cellular p...
Keywords: Western blot

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Peer-Reviewed Papers
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