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F3777 Sigma

Monoclonal Anti-Actin, α-Smooth Muscle - FITC antibody produced in mouse

clone 1A4, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-Actin, α-Smooth Muscle, SMA


Related Categories AC-AC, Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology,
species reactivity   rabbit, mouse, guinea pig, chicken, goat, sheep, snake, human, frog, rat, canine, bovine
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human tonsil or appendix
clone   1A4, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
mol wt   antigen mol wt ~42 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)
biological source   mouse
conjugate   FITC conjugate



N-terminal synthetic decapeptide of α-smooth muscle actin.

General description

The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide, as a preservative.


Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.

Monoclonal Anti-Actin, α-Smooth Muscle - FITC antibody is suitable for:
• immunohistochemical (IHC) staining of heart, lung, kidney, and liver of mice to examine the PHD2 deficiency in internal organs
• immunostaining to analyze vascular smooth muscle cells/pericytes development in platelet-derived growth factor (PDGFR) and PDGFR- β-/- embryos
• immunofluorescent analysis of implanted cells, frozen tissue samples to study the similar levels of expression of SMaA and SM-MHC as established primary SMC lines
• western blots of lens epithelial cells to determine the distribution of α-SMA expression in lens epithelial cell

Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

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Biochem/physiol Actions

Actin, a conserved eukaryotic protein, is the major cytoskeletal component which actively participates in cell motility. In mammals and birds, actin is expressed as six different isoforms. α-Smooth muscle actin, a smooth muscle cell-specific protein, is also known as aortic smooth muscle or α smooth muscle actin (α-SMA, SMactin, α-SM-actin, ASMA). It belongs to actin family, which are highly conserved proteins that play a role in cell motility, structure and integrity. It is predominantly found in cells of the smooth muscle lineages. It has been reported that α-smooth muscle actin was also found in certain non-muscle cells, in particular fibroblasts i.e. myofibroblasts.

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