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F3899 Sigma

Monoclonal Anti-CD19−FITC antibody produced in mouse

clone SJ25-C1, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-CD19

Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood,
application(s)   flow cytometry: 10 μL using 1 × 106 cells
clone   SJ25-C1, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... CD19(930)
biological source   mouse
conjugate   FITC conjugate

Description

Immunogen

NALM-1 human cell line.

Antigen Background

CD19 is a member of the immunoglobulin superfamily and has two Ig-like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of κ or λ light chains. It is expressed on approximately 10% of normal human peripheral blood cells and approximately 60% of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

Recognizes the human CD19 (90 kDa) integral membrane glycoprotein.
3rd Workshop: code no. 073

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

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