Anti-Mouse IgG (Fab specific)−FITC antibody produced in goat

Features and Benefits

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Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Immunogen

Purified mouse IgG Fab fragment

Preparation Note

Adsorbed to reduce background staining with bovine, horse, or human samples.

Specificity

Binds all mouse Igs.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-Mouse IgG (Fab specific)-FITC antibody may be used for immunofluorescence of human peripheral blood lymphocytes at a minimum dilution of 1:64. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil slides at a working antibody dilution of 1:200. The antibody was used at a dilution range of 1:40 to 1:200 for immunofluorescence in various studies.^1,2,3,4

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.⁵ Anti-Mouse IgG (Fab specific)-FITC antibody is specific for Fab fragment of all mouse IgG subclasses. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC. The antibody is adsorbed with human IgG to ensure minimal cross reactivity. The conjugate shows no reactivity with mouse IgG, Fc fragment, human, bovine, or horse serum.