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F4024 Sigma

Monoclonal Anti-Biotin–FITC antibody produced in mouse

purified immunoglobulin, buffered aqueous solution, clone BN-34

Synonym: Monoclonal Anti-Biotin

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies to Biotin, B-BI, Biotin and Biotin Antibodies,
biological source   mouse
antibody form   purified immunoglobulin
clone   BN-34, monoclonal
form   buffered aqueous solution
storage condition   protect from light
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80
isotype   IgG1
conjugate   FITC conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Features and Benefits

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Immunogen

Biotin-KLH conjugate

Physical form

Solution in phosphate buffered saline, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

General description

The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immuno­histo­chemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Protocols & Articles

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Peer-Reviewed Papers
15

References

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