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F4042 Sigma

Monoclonal ANTI-FLAG® M5 antibody produced in mouse

clone M5, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-ddddk, Anti-dykddddk



Related Categories Alphabetical Index, Antibodies, Antibodies to Peptide and Protein Fusion Tags, FK-FO, FLAG Antibodies,
biological source   mouse
antibody form   purified immunoglobulin
clone   M5, monoclonal
form   buffered aqueous solution
concentration   2-5 mg/mL
application(s)   western blot (chemiluminescent): 10 μg/mL
isotype   IgG1
immunogen sequence   DYKDDDDK
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C



Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.

Suitable for western blotting.

Browse additional application references in our FLAG® Literature portal.

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General description

Monoclonal ANTI-FLAG® M5 is a mouse antibody that binds to N-terminal Met-FLAG fusion proteins. It is useful for detection of N-terminal Met-FLAG fusion proteins expressed in mammalian and Drosophilae cells.


FLAG; peptide sequence DYKDDDDK

Other Notes

Antibody is not calcium dependent.

Preparation Note

Dilute the antibody to 10 mg/mL in Tris buffered saline (TBS): 0.05 M Tris, 0.15 M NaCl, pH 7.4.

Physical form

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

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Safety Information

NONH for all modes of transport
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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